ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANSPKKPSD GTGVSASDTP KYQHTVPETK PAFNLSPGKA SELSHSLPSP SQIKSTAHVS 

        70         80         90        100        110        120 
STHNDAAGNT DDSVLPKNVS PTTNLRVESN GDTNNMFSSP AGLALPKKDD KKKNKGTSKA 

       130        140        150        160        170        180 
DSKDGKASNS SGQNAQQQSD PNKMQDVLFS AGIDVREEEA LLNSSINASK SQVQTNNVKI 

       190        200        210        220        230        240 
PNHLPFLHPE QVSNYMRKVG KEQNFNLTPT KNPEILDMMS SACENYMRDI LTNAIVISRH 

       250        260        270        280        290        300 
RRKAVKINSG RRSEVSAALR AIALIQKKEE ERRVKKRIAL GLEKEDYENK IDSEETLHRA 

       310        320        330        340        350        360 
SNVTAGLRAG SKKQYGWLTS SVNKPTSLGA KSSGKVASDI TARGESGLKF REAREEPGIV 

       370        380 
MRDLLFALEN RRNSVQTIIS KGYAKIRD 

References and documentation are available.

Number of amino acids: 388

Molecular weight: 42336.47

Theoretical pI: 9.67

Amino acid composition: 
Ala (A)  32	  8.2%
Arg (R)  22	  5.7%
Asn (N)  30	  7.7%
Asp (D)  20	  5.2%
Cys (C)   1	  0.3%
Gln (Q)  14	  3.6%
Glu (E)  24	  6.2%
Gly (G)  22	  5.7%
His (H)   8	  2.1%
Ile (I)  18	  4.6%
Leu (L)  27	  7.0%
Lys (K)  38	  9.8%
Met (M)   8	  2.1%
Phe (F)   7	  1.8%
Pro (P)  20	  5.2%
Ser (S)  48	 12.4%
Thr (T)  21	  5.4%
Trp (W)   1	  0.3%
Tyr (Y)   6	  1.5%
Val (V)  21	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      1812
Hydrogen    H	      2979
Nitrogen    N	       553
Oxygen      O	       596
Sulfur      S	         9

Formula: C1812H2979N553O596S9
Total number of atoms: 5949

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.341, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.341, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.14
This classifies the protein as unstable.



Aliphatic index: 69.18

Grand average of hydropathicity (GRAVY): -0.816