ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANVDEGGRR RPRLAASLQI SPGPWRPSGG QESTEVEDGA PGTDESGVAR EQEVHGEACK 

        70         80         90        100        110        120 
ALEGSVLSPG PAGDFPMALR GLSLLPKDPS PAQAMALLTQ YMANLGVSLT FLEDQTADPG 

       130        140        150        160        170        180 
SSFSVCAELD GLVCPAGTGS SKLEAKQQAA LSALQYIQKQ LERPVMKFKR SCLPCPYVRT 

       190        200        210        220        230        240 
TLHLFAGPLV APSDPVPSTC HGLSLNWSLG DPDIEVVDVA TGRVKTDSSV GPPSRLCKAA 

       250        260        270        280        290        300 
FLAAFRQVAR ALEKPQLLSL QTYEAAKAIP YREARQQLSL LLDQQGLGAW PSKPLVGKFR 


H 

References and documentation are available.

Number of amino acids: 301

Molecular weight: 31995.53

Theoretical pI: 6.45

Amino acid composition: 
Ala (A)  34	 11.3%
Arg (R)  17	  5.6%
Asn (N)   3	  1.0%
Asp (D)  14	  4.7%
Cys (C)   7	  2.3%
Gln (Q)  18	  6.0%
Glu (E)  17	  5.6%
Gly (G)  26	  8.6%
His (H)   4	  1.3%
Ile (I)   4	  1.3%
Leu (L)  38	 12.6%
Lys (K)  13	  4.3%
Met (M)   5	  1.7%
Phe (F)   8	  2.7%
Pro (P)  27	  9.0%
Ser (S)  27	  9.0%
Thr (T)  12	  4.0%
Trp (W)   3	  1.0%
Tyr (Y)   5	  1.7%
Val (V)  19	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1408
Hydrogen    H	      2257
Nitrogen    N	       397
Oxygen      O	       429
Sulfur      S	        12

Formula: C1408H2257N397O429S12
Total number of atoms: 4503

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24325
Abs 0.1% (=1 g/l)   0.760, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.749, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.71
This classifies the protein as unstable.



Aliphatic index: 84.02

Grand average of hydropathicity (GRAVY): -0.206