ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANVEKPNDC SGFPVVDLNS CFSNGFNNEK QEIEMETDDS PILLMSSSAS RENSNTFSVI 

        70         80         90        100        110        120 
QRTPDGKIIT TNNNMNSKIN KQLDKLPENL RLNGRTPSGK LRSFVCEVCT RAFARQEHLK 

       130        140        150        160        170        180 
RHYRSHTNEK PYPCGLCNRC FTRRDLLIRH AQKIHSGNLG ETISHTKKVS RTITKARKNS 

       190        200        210        220        230        240 
ASSVKFQTPT YGTPDNGNFL NRTTANTRRK ASPEANVKRK YLKKLTRRAS FSAQSASSYA 

       250        260        270        280        290        300 
LPDQSSLEQH PKDRVKFSTP ELVPLDLKNP ELDSSFDLNM NLDLNLNLDS NFNIALNRSD 

       310        320        330        340        350        360 
SSGSTMNLDY KLPESANNYT YSSGSPTRAY VGANTNSKNA SFNDADLLSS SYWIKAYNDH 

       370        380        390        400        410        420 
LFSVSESDET SPMNSELNDT KLIVPDFKST IHHLKDSRSS SWTVAIDNNS NNNKVSDNQP 

       430        440        450        460        470        480 
DFVDFQELLD NDTLGNDLLE TTAVLKEFEL LHDDSVSATA TSNEIDLSHL NLSNSPISPH 

       490        500        510        520        530        540 
KLIYKNKEGT NDDMLISFGL DHPSNREDDL DKLCNMTRDV QAIFSQYLKG EESKRSLEDF 

       550        560        570        580        590        600 
LSTSNRKEKP DSGNYTFYGL DCLTLSKISR ALPASTVNNN QPSHSIESKL FNEPMRNMCI 

       610        620        630        640        650        660 
KVLRYYEKFS HDSSESVMDS NPNLLSKELL MPAVSELNEY LDLFKNNFLP HFPIIHPSLL 

       670        680        690        700        710        720 
DLDLDSLQRY TNEDGYDDAE NAQLFDRLSQ GTDKEYDYEH YQILSISKIV CLPLFMATFG 

       730        740        750        760        770        780 
SLHKFGYKSQ TIELYEMSRR ILHSFLETKR RCRSTTVNDS YQNIWLMQSL ILSFMFALVA 

       790        800        810        820        830        840 
DYLEKIDSSL MKRQLSALCS TIRSNCLPTI SANSEKSINN NNEPLTFGSP LQYIIFESKI 

       850        860        870        880        890        900 
RCTLMAYDFC QFLKCFFHIK FDLSIKEKDV ETIYIPDNES KWASESIICN GHVVQKQNFY 

       910        920        930        940        950        960 
DFRNFYYSFT YGHLHSIPEF LGSSMIYYEY DLRKGTKSHV FLDRIDTKRL ERSLDTSSYG 

       970        980        990       1000       1010       1020 
NDNMAATNKN IAILIDDTII LKNNLMSMRF IKQIDRSFTE KVRKGQIAKI YDSFLNSVRL 

      1030       1040       1050       1060       1070       1080 
NFLKNYSVEV LCEFLVALNF SIRNISSLYV EEESDCSQRM NSPELPRIHL NNQALSVFNL 

      1090       1100       1110       1120       1130       1140 
QGYYYCFILI IKFLLDFEAT PNFKLLRIFI ELRSLANSIL LPTLSRLYPQ EFSGFPDVVF 

      1150       1160       1170       1180       1190       1200 
TQQFINKDNG MLVPGLSANE HHNGASAAVK TKLAKKINVE GLAMFINEIL VNSFNDTSFL 

      1210       1220       1230       1240       1250       1260 
NMEDPIRNEF SFDNGDRAVT DLPRSAHFLS DTGLEGINFS GLNDSHQTVS TLNLLRYGEN 

      1270       1280       1290       1300       1310       1320 
HSSKHKNGGK GQGFAEKYQL SLKYVTIAKL FFTNVKENYI HCHMLDKMAS DFHTLENHLK 


GNS 

References and documentation are available.

Number of amino acids: 1323

Molecular weight: 150940.92

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  57	  4.3%
Arg (R)  61	  4.6%
Asn (N) 120	  9.1%
Asp (D)  86	  6.5%
Cys (C)  22	  1.7%
Gln (Q)  37	  2.8%
Glu (E)  75	  5.7%
Gly (G)  46	  3.5%
His (H)  37	  2.8%
Ile (I)  74	  5.6%
Leu (L) 147	 11.1%
Lys (K)  86	  6.5%
Met (M)  29	  2.2%
Phe (F)  75	  5.7%
Pro (P)  48	  3.6%
Ser (S) 151	 11.4%
Thr (T)  71	  5.4%
Trp (W)   4	  0.3%
Tyr (Y)  49	  3.7%
Val (V)  48	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 161
Total number of positively charged residues (Arg + Lys): 147

Atomic composition:

Carbon      C	      6665
Hydrogen    H	     10397
Nitrogen    N	      1827
Oxygen      O	      2074
Sulfur      S	        51

Formula: C6665H10397N1827O2074S51
Total number of atoms: 21014

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    96385
Abs 0.1% (=1 g/l)   0.639, assuming all pairs of Cys residues form cystines


Ext. coefficient    95010
Abs 0.1% (=1 g/l)   0.629, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.58
This classifies the protein as unstable.



Aliphatic index: 79.98

Grand average of hydropathicity (GRAVY): -0.498