ProtParam
User-provided sequence:
10 20 30 40 50 60
MANYTKTAIL SVYDKTGLLD LARGLIEKNV RILASGGTAR MIRDAGFPIE DVSAITHAPE
70 80 90 100 110 120
MLGGRVKTLH PAVHGGILAR DIDSDEKDLK EQHIEKVDYV VCNLYPFKET VAKVGVTIPE
130 140 150 160 170 180
AVEEIDIGGV TLLRAAAKNH ARVTILSDPK DYSEFLSELS SNGEISQDLR NRLALKAFEH
190 200 210 220 230 240
TADYDAAISD FFRKQYSEGQ AQITLRYGAN PHQKPAQAYV SQQDSLPFKV LCGSPGYINL
250 260 270 280 290 300
LDALNSWPLV KELSASLNLP AAASFKHVSP AGAAVGIPLS DVEKQVYFVA DIENLSPLAC
310 320 330 340 350 360
AYARARGADR MSSFGDWIAL SNIVDVPTAK IISREVSDGV IAPGYEPEAL AILSKKKGGK
370 380 390 400 410 420
YCILQIDPNY VPEAVERRQV YGVTLEQKRN DAIINQSTFK EIVSQNKNLT EQAIIDLTVA
430 440 450 460 470 480
TIAIKYTQSN SVCYARNGMV VGLGAGQQSR IHCTRLAGDK ADNWWFRQHP RVLEIKWAKG
490 500 510 520 530 540
VKRPEKSNAI DLFVTGQIPT EEPELSEYQS KFEEIPKPFT PEERKEWLSK LTNVSLSSDA
550 560 570 580 590
FFPFPDNVYR AVKSGVKYIA APSGSVMDKV VFSAADSFDL VYVENPIRLF HH
References and
documentation are available.
Number of amino acids: 592
Molecular weight: 65263.31
Theoretical pI: 6.12
Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 67
Atomic composition:
Carbon C 2925
Hydrogen H 4615
Nitrogen N 791
Oxygen O 876
Sulfur S 12
Formula: C
2925H
4615N
791O
876S
12
Total number of atoms: 9219
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66155
Abs 0.1% (=1 g/l) 1.014, assuming all pairs of Cys residues form cystines
Ext. coefficient 65780
Abs 0.1% (=1 g/l) 1.008, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.14
This classifies the protein as unstable.
Aliphatic index: 92.62
Grand average of hydropathicity (GRAVY): -0.203