ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPAVGIDLG TTYSCVGIFR DDRIEIIAND QGNRTTPSFV AFTDTERLIG DAAKNQVAMN 

        70         80         90        100        110        120 
PINTVFDAKR LIGRKFNDAE VQADMKHFPF KVVEKSGKPI VQVEFKGEEK QFTPEEISSM 

       130        140        150        160        170        180 
VLTKMRETAE AYLGGTVNNA VITVPAYFND SQRQATKDAG LIAGLNVLRI INEPTAGGIA 

       190        200        210        220        230        240 
YGLDKKADGE RNVLIFDFGG GTFDVSLLTI EEGIFEVKST AGDTQLGGEE FDNRLVNHFV 

       250        260        270        280        290        300 
NEFKRKNKKD LSTNARALRR LRTACERAKR TLSSAAQTSI EIDSLYEGVD FYTSITRARF 

       310        320        330        340        350        360 
EELCQDLFRS TMEPVERVLR DAKIDKSSVH EIVLVGGSTR IPKIQKMVSD FFNGKEPNKS 

       370        380        390        400        410        420 
INPDEGVAYG AAVQAAILSG DTSSKSTNEI LLLDVAPLSL GIETAGGVMT PLIKRNTTIP 

       430        440        450        460        470        480 
TKKSETFSTF SDNQPGVLIQ VFEGERARTK DNNLLGKFEL TGIPPAPRGV PQIEVTFDVD 

       490        500        510        520        530        540 
ANGIMNVSAL EKGTGKTNKI VITNDKGRLS KEEIERMLAE AEKYKAEDEA ETARIGAKNG 

       550        560        570        580        590        600 
LESYAYSLKN TLSDSKVDEK LDAADKEKLK SEIDKVVAWL DDNQTATKEE YESQQKELEG 

       610        620        630        640        650 
VANPIMMKFY GAGGEGGAPG GFPGAGAGGP GGFPGAGAGG AAAHDDGPTV EEVD 

References and documentation are available.

Number of amino acids: 654

Molecular weight: 70733.59

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)  61	  9.3%
Arg (R)  31	  4.7%
Asn (N)  33	  5.0%
Asp (D)  43	  6.6%
Cys (C)   3	  0.5%
Gln (Q)  18	  2.8%
Glu (E)  57	  8.7%
Gly (G)  63	  9.6%
His (H)   4	  0.6%
Ile (I)  41	  6.3%
Leu (L)  44	  6.7%
Lys (K)  49	  7.5%
Met (M)  12	  1.8%
Phe (F)  30	  4.6%
Pro (P)  26	  4.0%
Ser (S)  36	  5.5%
Thr (T)  46	  7.0%
Trp (W)   1	  0.2%
Tyr (Y)  12	  1.8%
Val (V)  44	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      3102
Hydrogen    H	      4966
Nitrogen    N	       856
Oxygen      O	      1000
Sulfur      S	        15

Formula: C3102H4966N856O1000S15
Total number of atoms: 9939

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23505
Abs 0.1% (=1 g/l)   0.332, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.331, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.06
This classifies the protein as stable.



Aliphatic index: 79.53

Grand average of hydropathicity (GRAVY): -0.391