ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPELITIGA DHSILPAESL IPVDRAYVTF LAGNGDYVKG VVGLAKGLRK VKAAYPLVVA 

        70         80         90        100        110        120 
VLPDVPLEHR RLLEAQGCIV REIEPIYPPE NNCEFAHAYY VINYSKLRIW EFVEYSKMIY 

       130        140        150        160        170        180 
LDGDIQVYQN IDHLFDQPDG YFYAVMDCFC EPSWSKTIQY KIGYCQQCPE KVAWPLEAGP 

       190        200        210        220        230        240 
KPSLYFNAGF FVYEPSLETY KDLIDTLKVT TPTSFAEQDF LNMYFKDKFK PLPIDYNLVL 

       250        260        270        280        290        300 
AFLWRHPEKV DLNRVKVVHY CAAGSKPWRY TGKEENMDRE DIKLLVKKWW DIYNDESLDL 

       310        320        330 
KKPVHLVQQP TEVLKAALSE ARPVKYVAAP SAA 

References and documentation are available.

Number of amino acids: 333

Molecular weight: 38268.16

Theoretical pI: 5.60

Amino acid composition: 
Ala (A)  27	  8.1%
Arg (R)  11	  3.3%
Asn (N)  11	  3.3%
Asp (D)  21	  6.3%
Cys (C)   7	  2.1%
Gln (Q)  10	  3.0%
Glu (E)  23	  6.9%
Gly (G)  14	  4.2%
His (H)   7	  2.1%
Ile (I)  18	  5.4%
Leu (L)  32	  9.6%
Lys (K)  26	  7.8%
Met (M)   5	  1.5%
Phe (F)  14	  4.2%
Pro (P)  25	  7.5%
Ser (S)  13	  3.9%
Thr (T)  10	  3.0%
Trp (W)   7	  2.1%
Tyr (Y)  23	  6.9%
Val (V)  29	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1771
Hydrogen    H	      2688
Nitrogen    N	       434
Oxygen      O	       489
Sulfur      S	        12

Formula: C1771H2688N434O489S12
Total number of atoms: 5394

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73145
Abs 0.1% (=1 g/l)   1.911, assuming all pairs of Cys residues form cystines


Ext. coefficient    72770
Abs 0.1% (=1 g/l)   1.902, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.15
This classifies the protein as stable.



Aliphatic index: 91.92

Grand average of hydropathicity (GRAVY): -0.183