ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPFRQDSIL IIYPRSQTTL VQFGLNEETF TVPELEIPTQ IYRTTRQDGS YTYHSTNKDN
70 80 90 100 110 120
KAELIKPIQN GEIIDISAFT QFLRLIFVSI LSDRANKNQD AFEAELSNIP LLLITHHSWS
130 140 150 160 170 180
QSDLEIITQY VFESLEINNL IQLPASLAAT YSMISLQNCC IIDVGTHHTD IIPIVDYAQL
190 200 210 220 230 240
DHLVSSIPMG GQSINDSLKK LLPQWDDDQI ESLKKSPIFE VLSDDAKKLS SFDFGNENED
250 260 270 280 290 300
EDEGTLNVAE IITSGRDTRE VLEERERGQK VKNVKNSDLE FNTFWDEKGN EIKVGKQRFQ
310 320 330 340 350 360
GCNNLIKNIS NRVGLTLDNI DDINKAKAVW ENIIIVGGTT SISGFKEALL GQLLKDHLII
370 380 390 400 410 420
EPEEEKSKRE EEAKSVLPAA TKKKSKFMTN STAFVPTIEY VQCPTVIKLA KYPDYFPEWK
430 440 450 460
KSGYSEIIFL GAQIVSKQIF THPKDTFYIT REKYNMKGPA ALWDVQF
References and
documentation are available.
Number of amino acids: 467
Molecular weight: 53074.19
Theoretical pI: 5.03
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2382
Hydrogen H 3746
Nitrogen N 618
Oxygen O 734
Sulfur S 9
Formula: C
2382H
3746N
618O
734S
9
Total number of atoms: 7489
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52620
Abs 0.1% (=1 g/l) 0.991, assuming all pairs of Cys residues form cystines
Ext. coefficient 52370
Abs 0.1% (=1 g/l) 0.987, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.87
This classifies the protein as stable.
Aliphatic index: 92.91
Grand average of hydropathicity (GRAVY): -0.363