ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPIAGKKAK RGILERLNAG EVVIGDGGFV FALEKRGYVK AGPWTPEAAV EHPEAVRQLH 

        70         80         90        100        110        120 
REFLRAGSNV MQTFTFYASE DKLENRGNYV AEKISGQKVN EAACDIARQV ADEGDALVAG 

       130        140        150        160        170        180 
GVSQTPSYLS CKSETEVKKI FHQQLEVFMK KNVDFLIAEY FEHVEEAVWA VEALKTSGKP 

       190        200        210        220        230        240 
IAATMCIGPE GDLHGVSPGE CAVRLVKAGA AIVGVNCHFD PSTSLQTIKL MKEGLEAARL 

       250        260        270        280        290        300 
KAYLMSHALA YHTPDCGKQG FIDLPEFPFG LEPRVATRWD IQKYAREAYN LGVRYIGGCC 

       310        320        330        340        350        360 
GFEPYHIRAI AEELAPERGF LPPASEKHGS WGSGLDMHTK PWIRARARKE YWQNLRIASG 

       370        380        390        400 
RPYNPSMSKP DAWGVTKGAA ELMQQKEATT EQQLRALFEK QKFKSAQ 

References and documentation are available.

Number of amino acids: 407

Molecular weight: 44976.41

Theoretical pI: 8.02

Amino acid composition: 
Ala (A)  49	 12.0%
Arg (R)  22	  5.4%
Asn (N)  10	  2.5%
Asp (D)  13	  3.2%
Cys (C)   8	  2.0%
Gln (Q)  17	  4.2%
Glu (E)  37	  9.1%
Gly (G)  36	  8.8%
His (H)  11	  2.7%
Ile (I)  18	  4.4%
Leu (L)  29	  7.1%
Lys (K)  30	  7.4%
Met (M)   9	  2.2%
Phe (F)  17	  4.2%
Pro (P)  21	  5.2%
Ser (S)  19	  4.7%
Thr (T)  15	  3.7%
Trp (W)   7	  1.7%
Tyr (Y)  13	  3.2%
Val (V)  26	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2009
Hydrogen    H	      3135
Nitrogen    N	       559
Oxygen      O	       582
Sulfur      S	        17

Formula: C2009H3135N559O582S17
Total number of atoms: 6302

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58370
Abs 0.1% (=1 g/l)   1.298, assuming all pairs of Cys residues form cystines


Ext. coefficient    57870
Abs 0.1% (=1 g/l)   1.287, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.86
This classifies the protein as stable.



Aliphatic index: 75.60

Grand average of hydropathicity (GRAVY): -0.355