ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPKISISLN PPYNGEFYSS NDQMSGIVSL QLTKALSIRK ISVILKGFSE TLTKIDQEYM 

        70         80         90        100        110        120 
FQQNGMMMPG QDNKSFHTLM KFEQRVFPPD NVWNALDGSS KPFKVKPGSY NYSFQFDKFP 

       130        140        150        160        170        180 
RKPECLKNHT AKTVAFVTRS NARLPPTFNS HWQEFNKIDN LDLYFYSFGK VIYMVQVQLE 

       190        200        210        220        230        240 
LGKSSSWFKP FHKLIREIET FEFIPEPKDL IIEPDEDDNE ELNAFSNNSR GNSMVTNNEF 

       250        260        270        280        290        300 
FNSSNLKVPS KDVKVVNGVG YIKSDRNFSQ ANSILIENGD IRSRPVSSVT STRQSTRLVN 

       310        320        330        340        350        360 
GMKVFPSTYK MGLPDGESNM RIEVRSRDLK QIYRKDYLFR SGSQNFDKVY VVMEGNIASL 

       370        380        390        400        410        420 
SKMQITPLKL QLNLLETTTY LSQGIANGNY SSLKLIEIDL NQLKSNKPLL DLNEIRENFD 

       430        440        450        460        470        480 
GSMFECELRL KDHPILRKLV FNEEDYRHRG NRLYSFKTCT IKRTFSLQLL IEWGINGIRK 

       490        500        510 
QSEVNIDPVQ IFCQVREHVE AEALPRYVPP PTYTEMAS 

References and documentation are available.

Number of amino acids: 518

Molecular weight: 59758.18

Theoretical pI: 8.90

Amino acid composition: 
Ala (A)  13	  2.5%
Arg (R)  27	  5.2%
Asn (N)  41	  7.9%
Asp (D)  25	  4.8%
Cys (C)   4	  0.8%
Gln (Q)  23	  4.4%
Glu (E)  34	  6.6%
Gly (G)  24	  4.6%
His (H)   7	  1.4%
Ile (I)  33	  6.4%
Leu (L)  46	  8.9%
Lys (K)  39	  7.5%
Met (M)  16	  3.1%
Phe (F)  32	  6.2%
Pro (P)  28	  5.4%
Ser (S)  50	  9.7%
Thr (T)  23	  4.4%
Trp (W)   4	  0.8%
Tyr (Y)  19	  3.7%
Val (V)  30	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2675
Hydrogen    H	      4182
Nitrogen    N	       720
Oxygen      O	       793
Sulfur      S	        20

Formula: C2675H4182N720O793S20
Total number of atoms: 8390

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50560
Abs 0.1% (=1 g/l)   0.846, assuming all pairs of Cys residues form cystines


Ext. coefficient    50310
Abs 0.1% (=1 g/l)   0.842, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.40
This classifies the protein as unstable.



Aliphatic index: 78.78

Grand average of hydropathicity (GRAVY): -0.507