ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPKRKSVKG GVEPKNKKVK TAIHQKEPNT LLSLEELTNL SKEIDESLKF NNLVPLLEQF
70 80 90 100 110 120
KLIKTKLIES IDPDVESTAR KLSLLLYRNF NKINDEIISN GNGAKSSASE DKKMIIKWLS
130 140 150 160 170 180
EKYTTFKDII YGFIEDKLAG STSLQLDLLD IILNFIRLES QRKSSTSQVV FFPTQSYKSL
190 200 210 220 230 240
TESILNSKNG EISFDGTTDN FIVLEFLDKF QKYWDLQFYF FNNLSEVLNE WKQEKSEKDL
250 260 270 280 290 300
QLIFANFLTV LRKGLLFNEK TLEEEPLWIA PNKEGKLPSS LYKHTLFKSQ FQKSVIAMLS
310 320 330 340 350 360
YPLLQEQYKS VLTILHKRII PYMSQPQSLM DFLTDCYDLT DDLIVPILAL NSLYELMKNY
370 380 390 400 410 420
NLEYPDFYSK LYSLLTPELL YTKYRSRFFR LCDLFLSSTH LSANLVASFI KKLARISLAA
430 440 450 460 470 480
SASGVVIIIP FIYNLLKRHP TCMIMLHKED TESGYQDPFN SEEKNPLHTE AIKSSLWELD
490 500 510 520 530 540
TLMTHYHPNV ATLAKIFGEP FRKPSYNMED FLDWSYNSLL QSEYDRKFKD KNSALEFEEF
550 560
DKVLDTSNSD SKSSLLEGWT LV
References and
documentation are available.
Number of amino acids: 562
Molecular weight: 65200.98
Theoretical pI: 6.10
Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 2984
Hydrogen H 4658
Nitrogen N 734
Oxygen O 873
Sulfur S 13
Formula: C
2984H
4658N
734O
873S
13
Total number of atoms: 9262
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 74385
Abs 0.1% (=1 g/l) 1.141, assuming all pairs of Cys residues form cystines
Ext. coefficient 74260
Abs 0.1% (=1 g/l) 1.139, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.18
This classifies the protein as unstable.
Aliphatic index: 95.94
Grand average of hydropathicity (GRAVY): -0.304