ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPKSSSSGS KKKSSASSNS ADAKASKFKL PAEFITRPHP SKDHGKETCT AYIHPNVLSS 

        70         80         90        100        110        120 
LEINPGSFCT VGKIGENGIL VIARAGDEEV HPVNVITLST TIRSVGNLIL GDRLELKKAQ 

       130        140        150        160        170        180 
VQPPYATKVT VGSLQGYNIL ECMEEKVIQK LLDDSGVIMP GMIFQNLKTK AGDESIDVVI 

       190        200        210        220        230        240 
TDASDDSLPD VSQLDLNMDD MYGGLDNLFY LSPPFIFRKG STHITFSKET QANRKYNLPE 

       250        260        270        280        290        300 
PLSYAAVGGL DKEIESLKSA IEIPLHQPTL FSSFGVSPPR GILLHGPPGT GKTMLLRVVA 

       310        320        330        340        350        360 
NTSNAHVLTI NGPSIVSKYL GETEAALRDI FNEARKYQPS IIFIDEIDSI APNRANDDSG 

       370        380        390        400        410        420 
EVESRVVATL LTLMDGMGAA GKVVVIAATN RPNSVDPALR RPGRFDQEVE IGIPDVDARF 

       430        440        450        460        470        480 
DILTKQFSRM SSDRHVLDSE AIKYIASKTH GYVGADLTAL CRESVMKTIQ RGLGTDANID 

       490        500        510        520        530        540 
KFSLKVTLKD VESAMVDIRP SAMREIFLEM PKVYWSDIGG QEELKTKMKE MIQLPLEASE 

       550        560        570        580        590        600 
TFARLGISAP KGVLLYGPPG CSKTLTAKAL ATESGINFLA VKGPEIFNKY VGESERAIRE 

       610        620        630        640        650        660 
IFRKARSAAP SIIFFDEIDA LSPDRDGSST SAANHVLTSL LNEIDGVEEL KGVVIVAATN 

       670        680        690        700        710        720 
RPDEIDAALL RPGRLDRHIY VGPPDVNARL EILKKCTKKF NTEESGVDLH ELADRTEGYS 

       730        740        750        760        770        780 
GAEVVLLCQE AGLAAIMEDL DVAKVELRHF EKAFKGIARG ITPEMLSYYE EFALRSGSSS 

References and documentation are available.

Number of amino acids: 780

Molecular weight: 84747.76

Theoretical pI: 5.49

Amino acid composition: 
Ala (A)  66	  8.5%
Arg (R)  38	  4.9%
Asn (N)  28	  3.6%
Asp (D)  49	  6.3%
Cys (C)   7	  0.9%
Gln (Q)  15	  1.9%
Glu (E)  57	  7.3%
Gly (G)  57	  7.3%
His (H)  14	  1.8%
Ile (I)  57	  7.3%
Leu (L)  73	  9.4%
Lys (K)  51	  6.5%
Met (M)  18	  2.3%
Phe (F)  27	  3.5%
Pro (P)  42	  5.4%
Ser (S)  69	  8.8%
Thr (T)  41	  5.3%
Trp (W)   1	  0.1%
Tyr (Y)  18	  2.3%
Val (V)  52	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 106
Total number of positively charged residues (Arg + Lys): 89

Atomic composition:

Carbon      C	      3746
Hydrogen    H	      6037
Nitrogen    N	      1017
Oxygen      O	      1164
Sulfur      S	        25

Formula: C3746H6037N1017O1164S25
Total number of atoms: 11989

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32695
Abs 0.1% (=1 g/l)   0.386, assuming all pairs of Cys residues form cystines


Ext. coefficient    32320
Abs 0.1% (=1 g/l)   0.381, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.72
This classifies the protein as unstable.



Aliphatic index: 92.79

Grand average of hydropathicity (GRAVY): -0.174