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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPKTIFITG ANRGIGLGLV RELLKVPGVE ALVAGARNID GAKELQSLAK ADARLHLIAV

70 80 90 100 110 120
DVSNDGSLEN SVKSVSGIVG DRGLNLLINN AGLIESYGTT SAPNRASVLH CIDVNAVSAL

130 140 150 160 170 180
LASQHFLPLL QKAASHVSGD SLTPDRAAIV NIGSDCASQA LNLRGSGPSN SLLAYKMSKV

190 200 210 220 230 240
AMLSFSRSMA ADFKRLEIPV LITNIHPGWV QTDMGGSNAE ISVDESVTKI VASIAKLNGG

250
HQGGLFNREL EEMPL


References and documentation are available.

Number of amino acids: 255 Molecular weight: 26598.50 Theoretical pI: 6.59
Amino acid composition: 
Ala (A) 29 11.4% Arg (R) 11 4.3% Asn (N) 16 6.3% Asp (D) 12 4.7% Cys (C) 2 0.8% Gln (Q) 6 2.4% Glu (E) 11 4.3% Gly (G) 24 9.4% His (H) 6 2.4% Ile (I) 17 6.7% Leu (L) 32 12.5% Lys (K) 11 4.3% Met (M) 6 2.4% Phe (F) 5 2.0% Pro (P) 9 3.5% Ser (S) 27 10.6% Thr (T) 8 3.1% Trp (W) 1 0.4% Tyr (Y) 2 0.8% Val (V) 20 7.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 23 Total number of positively charged residues (Arg + Lys): 22 Atomic composition: Carbon C 1162 Hydrogen H 1916 Nitrogen N 334 Oxygen O 361 Sulfur S 8 Formula: C1162H1916N334O361S8 Total number of atoms: 3781 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 8605 Abs 0.1% (=1 g/l) 0.324, assuming all pairs of Cys residues form cystines Ext. coefficient 8480 Abs 0.1% (=1 g/l) 0.319, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 32.47 This classifies the protein as stable. Aliphatic index: 109.06 Grand average of hydropathicity (GRAVY): 0.160