ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPKTIFITG ANRGIGLGLV RELLKVPGVE ALVAGARNID GAKELQSLAK ADARLHLIAV
70 80 90 100 110 120
DVSNDGSLEN SVKSVSGIVG DRGLNLLINN AGLIESYGTT SAPNRASVLH CIDVNAVSAL
130 140 150 160 170 180
LASQHFLPLL QKAASHVSGD SLTPDRAAIV NIGSDCASQA LNLRGSGPSN SLLAYKMSKV
190 200 210 220 230 240
AMLSFSRSMA ADFKRLEIPV LITNIHPGWV QTDMGGSNAE ISVDESVTKI VASIAKLNGG
250
HQGGLFNREL EEMPL
References and
documentation are available.
Number of amino acids: 255
Molecular weight: 26598.50
Theoretical pI: 6.59
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1162
Hydrogen H 1916
Nitrogen N 334
Oxygen O 361
Sulfur S 8
Formula: C
1162H
1916N
334O
361S
8
Total number of atoms: 3781
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8605
Abs 0.1% (=1 g/l) 0.324, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.319, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.47
This classifies the protein as stable.
Aliphatic index: 109.06
Grand average of hydropathicity (GRAVY): 0.160