ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPLTKKTNG KRSAKEVSHS EKKLAKKPRI SIDSSDEESE LSKKEDAVSS SSDDDDLDDL 

        70         80         90        100        110        120 
STSDSEAEEE ADELDISDDS EEHENENEEK EGKDKSEGGE NGNHTEQRKL LKERKMQRKS 

       130        140        150        160        170        180 
GTQVQQIKSV WERLRVKTPP LPKQIREKLS NEIWELSKDC ISDLVLKHDA SRIVQTLVKY 

       190        200        210        220        230        240 
SSKDRREQIV DALKGKFYVL ATSAYGKYLL VKLLHYGSRS SRQTIINELH GSLRKLMRHR 

       250        260        270        280        290        300 
EGAYVVEDLF VLYATHEQRQ QMIKEFWGSE YAVFRETHKD LTIEKVCESS IEKRNIIARN 

       310        320        330        340        350        360 
LIGTITASVE KGSTGFQILH AAMREYVKIA NEKEISEMIE LLHEQFAELV HTPEGSDVAC 

       370        380        390        400        410        420 
TLVARANAKE RKLILKALKN HAEKLIKNEY GNIVFITILN CVDDTVLVFK TFSPTVKEHL 

       430        440        450        460        470        480 
QEFIIDKFGR RPWLYILLGL DGKYFSPIVK NELLRYIELS KATSKKDPLQ RRHELLSKFA 

       490        500        510        520        530        540 
PMFLSTISKD YSSILTENLG CQFIAEVLIN DELYAQLNEK DQEKYQQVLN NILTTFKGDI 

       550        560        570        580        590        600 
TEEEHPIHRA FSTRLLKALI QGGKWNNKEK KVIPLKNVQG LGVPFAEKLY DEIIDSSNLL 

       610        620        630        640        650 
EWINNADSSF TIVALYETLK DQKEGKPFLK DLRGVQSKIT TDESNKGSQL LAKLLK 

References and documentation are available.

Number of amino acids: 656

Molecular weight: 75105.56

Theoretical pI: 6.44

Amino acid composition: 
Ala (A)  35	  5.3%
Arg (R)  31	  4.7%
Asn (N)  28	  4.3%
Asp (D)  38	  5.8%
Cys (C)   5	  0.8%
Gln (Q)  26	  4.0%
Glu (E)  69	 10.5%
Gly (G)  29	  4.4%
His (H)  17	  2.6%
Ile (I)  46	  7.0%
Leu (L)  74	 11.3%
Lys (K)  71	 10.8%
Met (M)   7	  1.1%
Phe (F)  20	  3.0%
Pro (P)  15	  2.3%
Ser (S)  55	  8.4%
Thr (T)  32	  4.9%
Trp (W)   6	  0.9%
Tyr (Y)  18	  2.7%
Val (V)  34	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      3332
Hydrogen    H	      5376
Nitrogen    N	       914
Oxygen      O	      1030
Sulfur      S	        12

Formula: C3332H5376N914O1030S12
Total number of atoms: 10664

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60070
Abs 0.1% (=1 g/l)   0.800, assuming all pairs of Cys residues form cystines


Ext. coefficient    59820
Abs 0.1% (=1 g/l)   0.796, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.23
This classifies the protein as unstable.



Aliphatic index: 91.71

Grand average of hydropathicity (GRAVY): -0.593