ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPNQRSRSL LQRHRGKVLI SLTGIAALFT TGSVVVFFVK RWLYKQQLRI TEQHFIKEQI
70 80 90 100 110 120
KRRFEQTQED SLYTIYELLP VWRMVLNEND LNLDSIVTQL KDQKNQLTRA KSSESRESSP
130 140 150 160 170 180
LKSKAELWNE LELKSLIKLV TVTYTVSSLI LLTRLQLNIL TRNEYLDSAI KLTMQQENCN
190 200 210 220 230 240
KLQNRFYNWV TSWWSDPEDK ADDAMVMAAK KSKKEGQEVY INEQAFLSLS WWILNKGWLS
250 260 270 280 290 300
YNEIITNQIE IEFDGIHPRD TLTLEEFSSR LTNIFRNTNS QIFQQNNNNL TSILLPKDSS
310 320 330 340 350 360
GQEFLLSQTL DADALTSFHS NTLVFNQLVN ELTQCIESTA TSIVLESLIN ESFHFIMNKV
370 380 390 400 410 420
GIKTIAKKKP GQEDQQQYQM AVFAMSMKDC CQEMLQTTAG SSHSGSVNEY LATLDSVQPL
430 440
DDLSASVYSN FGVSSSFSFK P
References and
documentation are available.
Number of amino acids: 441
Molecular weight: 50675.59
Theoretical pI: 5.98
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 2255
Hydrogen H 3566
Nitrogen N 604
Oxygen O 693
Sulfur S 14
Formula: C
2255H
3566N
604O
693S
14
Total number of atoms: 7132
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66140
Abs 0.1% (=1 g/l) 1.305, assuming all pairs of Cys residues form cystines
Ext. coefficient 65890
Abs 0.1% (=1 g/l) 1.300, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.29
This classifies the protein as unstable.
Aliphatic index: 91.29
Grand average of hydropathicity (GRAVY): -0.350