ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV 

        70         80         90        100        110        120 
ELKKGKLLKD GLHQYKKLIQ GSTEGLVSVG AVARKFIDLV ESKIASEQTR ADELQKQEID 

       130        140        150        160        170        180 
DDLEGGVTPE NLLISVYESD QSVAGFNDEA ITSWLRFTWE SYRAVLDLLR NNALLEITYS 

       190        200        210        220        230        240 
GVVKKTMHFC LKYQRKNEFK RLAEMLRQHL DAANYQQSKS GNNLVDLSDA DTLQRYLDQR 

       250        260        270        280        290        300 
FQQVDVSVKL ELWHEAYRSI EDVFHLMKIS KRAPKPSTLA NYYENLVKVF FVSGDPLLHT 

       310        320        330        340        350        360 
TAWKKFYKLY STNPRATEEE FKTYSSTIFL SAISTQLDEI PSIGYDPHLR MYRLLNLDAK 

       370        380        390        400        410        420 
PTRKEMLQSI IEDESIYGKV DEELKELYDI IEVNFDVDTV KQQLENLLVK LSSKTYFSQY 

       430        440        450        460        470        480 
IAPLRDVIMR RVFVAASQKF TTVSQSELYK LATLPAPLDL SAWDIEKSLL QAAVEDYVSI 

       490        500        510        520        530        540 
TIDHESAKVT FAKDPFDIFA STASKEVSEE ENTEPEVQEE KEETDEALGP QETEDGEEKE 

       550        560        570        580        590        600 
EESDPVIIRN SYIHNKLLEL SNVLHDVDSF NNASYMEKVR IARETLIKKN KDDLEKISKI 

       610        620        630        640        650        660 
VDERVKRSQE QKQKHMEHAA LHAEQDAEVR QQRILEEKAA IEAKLEEEAH RRLIEKKKRE 

       670        680        690        700        710        720 
FEAIKEREIT KMITEVNAKG HVYIDPNEAK SLDLDTIKQV IIAEVSKNKS ELESRMEYAM 

       730        740        750        760        770        780 
KKLDHTERAL RKVELPLLQK EVDKLQETDT ANYEAMKKKI VDAAKAEYEA RMADRKNLVM 

       790        800        810        820        830        840 
VYDDYLKFKE HVSGTKESEL AAIRNQKKAE LEAAKKARIE EVRKRRYEEA IARRKEEIAN 

       850        860        870        880        890        900 
AERQKRAQEL AEATRKQREI EEAAAKKSTP YSFRAGNREP PSTPSTLPKA TVSPDKAKLD 

       910        920        930        940        950        960 
MIAQKQREME EAIEQRLAGR TAGGSSPATP ATPATPATPT PSSGPKKMTM AEKLRAKRLA 


KGGR 

References and documentation are available.

Number of amino acids: 964

Molecular weight: 110344.39

Theoretical pI: 5.97

Amino acid composition: 
Ala (A)  92	  9.5%
Arg (R)  59	  6.1%
Asn (N)  30	  3.1%
Asp (D)  55	  5.7%
Cys (C)   1	  0.1%
Gln (Q)  42	  4.4%
Glu (E) 113	 11.7%
Gly (G)  26	  2.7%
His (H)  18	  1.9%
Ile (I)  54	  5.6%
Leu (L)  88	  9.1%
Lys (K)  96	 10.0%
Met (M)  19	  2.0%
Phe (F)  27	  2.8%
Pro (P)  36	  3.7%
Ser (S)  64	  6.6%
Thr (T)  51	  5.3%
Trp (W)   6	  0.6%
Tyr (Y)  31	  3.2%
Val (V)  56	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 168
Total number of positively charged residues (Arg + Lys): 155

Atomic composition:

Carbon      C	      4875
Hydrogen    H	      7861
Nitrogen    N	      1351
Oxygen      O	      1519
Sulfur      S	        20

Formula: C4875H7861N1351O1519S20
Total number of atoms: 15626

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    79190
Abs 0.1% (=1 g/l)   0.718, assuming all pairs of Cys residues form cystines


Ext. coefficient    79190
Abs 0.1% (=1 g/l)   0.718, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.02
This classifies the protein as unstable.



Aliphatic index: 83.84

Grand average of hydropathicity (GRAVY): -0.670