ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPPPLLPVA ASTPILHGEF GSYPANGPRF ALTLTTQALH IQRLRPKPEA RPRDGLVSLD
70 80 90 100 110 120
EVSGCGTLQS RSPEDTAAYF CIYTYPRGRR GGRRRATRTF RADGATTYEE NRAEAQRWAT
130 140 150 160 170 180
ALTCLLRGVP LSGDQEITPE LLPRKPRLLI LVNPFGGRGL AWQRCMDHVV PMISEAGLSF
190 200 210 220 230 240
NLIQTERQNH ARELVQGLSL SEWEGIVTVS GDGLLYEVLN GLLDRPDWED AVRMPIGVLP
250 260 270 280 290 300
CGSGNALAGA VNHHGGFEQV VGVDLLLNCS LLLCRGGSHP LDLLSVTLAS GSRCFSFLSV
310 320 330 340 350 360
AWGFLSDVDI HSERFRALGS ARFTLGAVLG LASLHTYRGR LSYLPATTEP ALPIPGHSLP
370 380 390 400 410 420
RAKSELVLAP APAPAATHSP LHRSVSDLPL PLPQPALVSP GSPEPLPDLS LNGGGPELTG
430 440 450 460 470 480
DWGGAGDAPL SPDPLLPSSP NALKTAQLSP IAEGPPEMPA SSGFLPPTHS APEASTWGPV
490 500 510 520 530 540
DHLLPPLGSP LPQDWVTIEG EFVLMLGILP SHLCADLMAA PHARFDDGVV HLCWVRSGIS
550 560 570 580 590 600
RAALLRILLA MEHGNHFSLG CPHLGYAAAR AFRLEPLTPR GLLTVDGELV EYGPIQAQVH
610
PGLATLLTGP AGQKPQA
References and
documentation are available.
Number of amino acids: 617
Molecular weight: 65618.20
Theoretical pI: 6.09
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2935
Hydrogen H 4629
Nitrogen N 825
Oxygen O 846
Sulfur S 19
Formula: C
2935H
4629N
825O
846S
19
Total number of atoms: 9254
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65025
Abs 0.1% (=1 g/l) 0.991, assuming all pairs of Cys residues form cystines
Ext. coefficient 64400
Abs 0.1% (=1 g/l) 0.981, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.24
This classifies the protein as unstable.
Aliphatic index: 95.56
Grand average of hydropathicity (GRAVY): -0.010