ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAPSIATVKI ARDMVLPLRI FVNRKQILQT NDKTSNKSNA TIFEAPLLSN NSIICLKSPN 

        70         80         90        100        110        120 
TRIYLSQQDK KNLCDEIKED LLLIVYELAS PEIISSVLSK IRVGHSTDFQ INVLPKLFAG 

       130        140        150        160        170        180 
ADTDNAVTSH IQSVTRLAKF KYKLHYKHKW ELDIFINSIK KIANLRHYLM FQTLTLNGFS 

       190        200        210        220        230        240 
LNAGPKTLLA RKIEKQPQVP NLLIENGDAD ALDTPVEEDI KPVIEFMYKP VINLGEIIDV 

       250        260 
HVLHRPRRHK VRTQSKQPQE E 

References and documentation are available.

Number of amino acids: 261

Molecular weight: 29769.65

Theoretical pI: 9.36

Amino acid composition: 
Ala (A)  15	  5.7%
Arg (R)  12	  4.6%
Asn (N)  17	  6.5%
Asp (D)  14	  5.4%
Cys (C)   2	  0.8%
Gln (Q)  12	  4.6%
Glu (E)  14	  5.4%
Gly (G)   6	  2.3%
His (H)   8	  3.1%
Ile (I)  26	 10.0%
Leu (L)  30	 11.5%
Lys (K)  24	  9.2%
Met (M)   4	  1.5%
Phe (F)   9	  3.4%
Pro (P)  14	  5.4%
Ser (S)  17	  6.5%
Thr (T)  14	  5.4%
Trp (W)   1	  0.4%
Tyr (Y)   6	  2.3%
Val (V)  16	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1340
Hydrogen    H	      2181
Nitrogen    N	       367
Oxygen      O	       384
Sulfur      S	         6

Formula: C1340H2181N367O384S6
Total number of atoms: 4278

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14565
Abs 0.1% (=1 g/l)   0.489, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.485, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.11
This classifies the protein as unstable.



Aliphatic index: 107.20

Grand average of hydropathicity (GRAVY): -0.255