ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPTISKRIK TLSVSRPIIY GNTAKKMGSV KPPNAPAEHT HLWTIFVRGP QNEDISYFIK
70 80 90 100 110 120
KVVFKLHDTY PNPVRSIEAP PFELTETGWG EFDINIKVYF VEEANEKVLN FYHRLRLHPY
130 140 150 160 170 180
ANPVPNSDNG NEQNTTDHNS KDAEVSSVYF DEIVFNEPNE EFFKILMSRP GNLLPSNKTD
190 200 210 220
DCVYSKQLEQ EEIDRIEIGI EKVDKEIDEL KQKLENLVKQ EAINGS
References and
documentation are available.
Number of amino acids: 226
Molecular weight: 25981.27
Theoretical pI: 5.26
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1166
Hydrogen H 1808
Nitrogen N 308
Oxygen O 357
Sulfur S 4
Formula: C
1166H
1808N
308O
357S
4
Total number of atoms: 3643
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.882, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.882, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.62
This classifies the protein as unstable.
Aliphatic index: 79.73
Grand average of hydropathicity (GRAVY): -0.648