ProtParam
User-provided sequence:
10 20 30 40 50 60
MAPTNLTKKP SQYKQSSRKG KKAWRKNIDL SDVEQYMEKK IDHEITHGTS DITSLQNDAL
70 80 90 100 110 120
FHVDVEGDEI LKNKLIKRKQ IKKVLKSKEI LDAVKTNSKI AALNHHKNSS GNPNKIQGVS
130 140 150 160 170 180
KHELKKLMAL AGRVHGESKI KNRVAKDGLV KTTAGDLWGE ESNSKKQKVK LPSGIKLDVE
190 200 210 220 230 240
KKDQIPEELL KKSTTSWSTA SVRPSTLDIE PIAVKEFTEI PHAGKSYNPN NKAWSELINK
250 260 270 280 290 300
EYKEEKARED ERIALEKYKE RIRHLMETLD DNEEEESSSN EEEEEEEEEN ENENESTQCS
310 320 330 340 350 360
GSDKEIKLSI NKPVKNKKKT KYQRNKAKRH EEKVKLQQEL KELRQRVKDL EEVINSEETE
370 380 390 400 410 420
ILSAIESDSN KVKKSKKNKK HKLGTKYSVI DERLEIKFSD ELSDSLRKLK PEGNLLYDTV
430 440 450
RKLQSSGKVE TRVPVRKGRK YKQKITEKWT HKDFK
References and
documentation are available.
Number of amino acids: 455
Molecular weight: 52555.63
Theoretical pI: 9.35
Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 100
Atomic composition:
Carbon C 2291
Hydrogen H 3792
Nitrogen N 666
Oxygen O 733
Sulfur S 5
Formula: C
2291H
3792N
666O
733S
5
Total number of atoms: 7487
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 0.778, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 0.778, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.88
This classifies the protein as unstable.
Aliphatic index: 73.47
Grand average of hydropathicity (GRAVY): -1.212