ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAQALNSTNI AFFRVAFLFT IAFFCLKNVN SILQNTYFIV LTQAMNLPQL TLSRYSGQLG 

        70         80         90        100        110        120 
LFALLFTLNG VHDLIPLLEN NVKYFQSVVP VRLLIFFILT SISYLWESNF YVHNNSVFIY 

       130        140        150        160        170        180 
CFAEVWINFL LYNAIREEKN EEFKRLNQFM VNDEDIEEPQ PFTVKTETTE IIEIINDEEN 

       190        200 
DDEDGKDNDD NNEKGNDDSD AKK 

References and documentation are available.

Number of amino acids: 203

Molecular weight: 23644.73

Theoretical pI: 4.34

Amino acid composition: 
Ala (A)  10	  4.9%
Arg (R)   5	  2.5%
Asn (N)  23	 11.3%
Asp (D)  13	  6.4%
Cys (C)   2	  1.0%
Gln (Q)   8	  3.9%
Glu (E)  17	  8.4%
Gly (G)   5	  2.5%
His (H)   2	  1.0%
Ile (I)  16	  7.9%
Leu (L)  23	 11.3%
Lys (K)   9	  4.4%
Met (M)   3	  1.5%
Phe (F)  19	  9.4%
Pro (P)   5	  2.5%
Ser (S)  10	  4.9%
Thr (T)  11	  5.4%
Trp (W)   2	  1.0%
Tyr (Y)   7	  3.4%
Val (V)  13	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 14

Atomic composition:

Carbon      C	      1080
Hydrogen    H	      1634
Nitrogen    N	       264
Oxygen      O	       323
Sulfur      S	         5

Formula: C1080H1634N264O323S5
Total number of atoms: 3306

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21555
Abs 0.1% (=1 g/l)   0.912, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.906, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.64
This classifies the protein as unstable.



Aliphatic index: 98.42

Grand average of hydropathicity (GRAVY): -0.090