ProtParam
User-provided sequence:
10 20 30 40 50 60
MAQEITHPTI VDGWFREISD TMWPGQAMTL KVEKVLHHEK SKYQDVLIFK STTYGNVLVL
70 80 90 100 110 120
DNVIQATERD EFAYQEMIAH LALNSHPNPK KVLVIGGGDG GVLREVVKHD SVEEAWLCDI
130 140 150 160 170 180
DEAVIRLSKE YLPEMAASYS HPKVKTHIGD GFQFLRDYQN TFDVIITDSS DPEGPAETLF
190 200 210 220 230 240
QKEYFQLLNS ALTEKGVITT QAESMWIHLP IIKDLKKACS EVFPVAEYSF VTIPTYPTGT
250 260 270 280 290
IGFMVCSKDK TCNVKKPLRE ISDEKEAELY RYYNKKIHEA SFVLPTWAAK ELN
References and
documentation are available.
Number of amino acids: 293
Molecular weight: 33324.06
Theoretical pI: 5.33
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1507
Hydrogen H 2334
Nitrogen N 382
Oxygen O 448
Sulfur S 11
Formula: C
1507H
2334N
382O
448S
11
Total number of atoms: 4682
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45630
Abs 0.1% (=1 g/l) 1.369, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.362, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.39
This classifies the protein as stable.
Aliphatic index: 86.48
Grand average of hydropathicity (GRAVY): -0.289