ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAQFSDIDKL AVSTLRLLSV DQVESAQSGH PGAPLGLAPV AHVIFKQLRC NPNNEHWINR 

        70         80         90        100        110        120 
DRFVLSNGHS CALLYSMLHL LGYDYSIEDL RQFRQVNSRT PGHPEFHSAG VEITSGPLGQ 

       130        140        150        160        170        180 
GISNAVGMAI AQANFAATYN EDGFPISDSY TFAIVGDGCL QEGVSSETSS LAGHLQLGNL 

       190        200        210        220        230        240 
ITFYDSNSIS IDGKTSYSFD EDVLKRYEAY GWEVMEVDKG DDDMESISSA LEKAKLSKDK 

       250        260        270        280        290        300 
PTIIKVTTTI GFGSLQQGTA GVHGSALKAD DVKQLKKRWG FDPNKSFVVP QEVYDYYKKT 

       310        320        330        340        350        360 
VVEPGQKLNE EWDRMFEEYK TKFPEKGKEL QRRLNGELPE GWEKHLPKFT PDDDALATRK 

       370        380        390        400        410        420 
TSQQVLTNMV QVLPELIGGS ADLTPSNLTR WEGAVDFQPP ITQLGNYAGR YIRYGVREHG 

       430        440        450        460        470        480 
MGAIMNGISA FGANYKPYGG TFLNFVSYAA GAVRLAALSG NPVIWVATHD SIGLGEDGPT 

       490        500        510        520        530        540 
HQPIETLAHL RAIPNMHVWR PADGNETSAA YYSAIKSGRT PSVVALSRQN LPQLEHSSFE 

       550        560        570        580        590        600 
KALKGGYVIH DVENPDIILV STGSEVSISI DAAKKLYDTK KIKARVVSLP DFYTFDRQSE 

       610        620        630        640        650        660 
EYRFSVLPDG VPIMSFEVLA TSSWGKYAHQ SFGLDEFGRS GKGPEIYKLF DFTADGVASR 

       670        680 
AEKTINYYKG KQLLSPMGRA F 

References and documentation are available.

Number of amino acids: 681

Molecular weight: 75029.41

Theoretical pI: 5.75

Amino acid composition: 
Ala (A)  53	  7.8%
Arg (R)  28	  4.1%
Asn (N)  26	  3.8%
Asp (D)  41	  6.0%
Cys (C)   3	  0.4%
Gln (Q)  27	  4.0%
Glu (E)  41	  6.0%
Gly (G)  61	  9.0%
His (H)  18	  2.6%
Ile (I)  35	  5.1%
Leu (L)  57	  8.4%
Lys (K)  40	  5.9%
Met (M)  12	  1.8%
Phe (F)  30	  4.4%
Pro (P)  34	  5.0%
Ser (S)  59	  8.7%
Thr (T)  34	  5.0%
Trp (W)   9	  1.3%
Tyr (Y)  29	  4.3%
Val (V)  44	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      3359
Hydrogen    H	      5179
Nitrogen    N	       903
Oxygen      O	      1021
Sulfur      S	        15

Formula: C3359H5179N903O1021S15
Total number of atoms: 10477

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    92835
Abs 0.1% (=1 g/l)   1.237, assuming all pairs of Cys residues form cystines


Ext. coefficient    92710
Abs 0.1% (=1 g/l)   1.236, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.41
This classifies the protein as stable.



Aliphatic index: 79.21

Grand average of hydropathicity (GRAVY): -0.351