ProtParam
User-provided sequence:
10 20 30 40 50 60
MAQMTMIQAI TDALRVEMKN DPNVLVFGED VGVNGGVFRA TEGLQAEFGE DRVMDTPLAE
70 80 90 100 110 120
SGIGGLAVGL ALEGFRPVPE IQFFGFVYEV MDSISGQLAR MRYRSGGRWT APVTVRSPFG
130 140 150 160 170 180
GGVHTPELHA DSLEGLVAQQ PGLKVVIPST PYDAKGLLIS AIRDNDPVIY LEHMKLYRSF
190 200 210 220 230 240
RQDVPEGEYT IDLGKADIKR EGTDVSVIAY GAMVHAALKA AEELEKEGIS LEVVDLRTVQ
250 260 270 280 290 300
PLDIETIIAS VEKTGRVVVV QEAQKQAGIA ANVVAEINDR AILNLEAPVV RVAAADTVFP
310 320
FSQAESVWLP NHKDIVEAVN KVMNF
References and
documentation are available.
Number of amino acids: 325
Molecular weight: 35229.28
Theoretical pI: 4.75
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1569
Hydrogen H 2503
Nitrogen N 423
Oxygen O 476
Sulfur S 10
Formula: C
1569H
2503N
423O
476S
10
Total number of atoms: 4981
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.608
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.45
This classifies the protein as stable.
Aliphatic index: 100.15
Grand average of hydropathicity (GRAVY): 0.053