ProtParam
User-provided sequence:
10 20 30 40 50 60
MAQNNFDFKF SQCFGDKADI VVTEADLITA VEFDYTGNYL ATGDKGGRVV LFERSNSRHC
70 80 90 100 110 120
EYKFLTEFQS HDAEFDYLKS LEIEEKINEI KWLRPTQRSH FLLSTNDKTI KLWKVYEKNI
130 140 150 160 170 180
KLVSQNNLTE GVTFAKKGKP DNHNSRGGSV RAVLSLQSLK LPQLSQHDKI IAATPKRIYS
190 200 210 220 230 240
NAHTYHINSI SLNSDQETFL SADDLRINLW NLDIPDQSFN IVDIKPTNME ELTEVITSAE
250 260 270 280 290 300
FHPQECNLFM YSSSKGTIKL CDMRQNSLCD NKTKTFEEYL DPINHNFFTE ITSSISDIKF
310 320 330 340 350 360
SPNGRYIASR DYLTVKIWDV NMDNKPLKTI NIHEQLKERL SDTYENDAIF DKFEVNFSGD
370 380 390 400 410 420
SSSVMTGSYN NNFMIYPNVV TSGDNDNGIV KTFDEHNAPN SNSNKNIHNS IQNKDSSSSG
430 440 450 460 470 480
NSHKRRSNGR NTGMVGSSNS SRSSIAGGEG ANSEDSGTEM NEIVLQADKT AFRNKRYGSL
490 500 510 520
AQRSARNKDW GDDIDFKKNI LHFSWHPREN SIAVAATNNL FIFSAL
References and
documentation are available.
Number of amino acids: 526
Molecular weight: 59662.19
Theoretical pI: 5.96
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 2614
Hydrogen H 4052
Nitrogen N 736
Oxygen O 839
Sulfur S 14
Formula: C
2614H
4052N
736O
839S
14
Total number of atoms: 8255
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 55600
Abs 0.1% (=1 g/l) 0.932, assuming all pairs of Cys residues form cystines
Ext. coefficient 55350
Abs 0.1% (=1 g/l) 0.928, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.20
This classifies the protein as unstable.
Aliphatic index: 71.20
Grand average of hydropathicity (GRAVY): -0.670