ProtParam
User-provided sequence:
10 20 30 40 50 60
MAQQEASPSP GAEVVGRAFV EQYYHILHQS PGLVHRFYQD SSFLTRPDVT GAVTTVTTMQ
70 80 90 100 110 120
AINDKILSLK YEDYTAEIET ADAQESHERG VIVLVTGRLT GNDNVRKKFS QSFFLAPQDK
130 140 150 160 170 180
GYFVLNDVFR FLEEKEVTAQ ARSVPINGTT RDVQAPIEPE RVVVSHEPEV EPEPVASIEE
190 200 210 220 230 240
EDLDNVAEVY DPSDKDEGVV VDVEPIEPPT QISHNEILSV PQGDAPKHSY ASILKQMKSS
250 260 270 280 290 300
PAPTTHVARN KPRPAPVNQK LTAPPAEPAA RPEASAHENV PNSSHVDVED DGHSIYVRNL
310 320 330 340 350 360
PFDSTPTQLE EVFKNFGAIK HEGIQVRSNK QQGFCFGFVE FETSSGKQSA LEASPVTIGD
370 380 390 400 410 420
RQAVVEEKKT NSRGGGNNGG SRGRYFSGRG SFRNESFKGG RGGGGRGGYG RGGGEFSGRP
430 440 450 460
KSSNPRNGGE GYQRVPQNGG GGRGGRGEGG RGGARGGGSS
References and
documentation are available.
Number of amino acids: 460
Molecular weight: 49543.40
Theoretical pI: 5.66
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2150
Hydrogen H 3355
Nitrogen N 641
Oxygen O 702
Sulfur S 4
Formula: C
2150H
3355N
641O
702S
4
Total number of atoms: 6852
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17880
Abs 0.1% (=1 g/l) 0.361, assuming all pairs of Cys residues form cystines
Ext. coefficient 17880
Abs 0.1% (=1 g/l) 0.361, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.51
This classifies the protein as unstable.
Aliphatic index: 60.57
Grand average of hydropathicity (GRAVY): -0.729