ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAQRFTDEVA IVTGSSNGIG RATAILLASE GAKVTITGRN AERLEESRQA LLKVGVPSGH 

        70         80         90        100        110        120 
INSVVADVTT GAGQDVLIDS TLKKFGKINI LINNAGALIV DPEGKTNTST GVETCLKTFQ 

       130        140        150        160        170        180 
LNFQSVVEMT QKIRPHLANT HGEIVNVSSV GAGPAAENRF PYYSAAKAAL DQYSRNTAID 

       190        200        210        220        230        240 
LIPDGIRVNI VQPGFVATGF TTAASGMSPD ASAKMYEGIG ANTSCIPAGY CGRPEHLASV 

       250        260        270        280 
IAFLADRKAS EYIVGQTIIA DGGTTLVRGR NVALATRRAE 

References and documentation are available.

Number of amino acids: 280

Molecular weight: 29263.18

Theoretical pI: 7.72

Amino acid composition: 
Ala (A)  36	 12.9%
Arg (R)  15	  5.4%
Asn (N)  15	  5.4%
Asp (D)  11	  3.9%
Cys (C)   3	  1.1%
Gln (Q)   9	  3.2%
Glu (E)  14	  5.0%
Gly (G)  28	 10.0%
His (H)   4	  1.4%
Ile (I)  22	  7.9%
Leu (L)  18	  6.4%
Lys (K)  11	  3.9%
Met (M)   4	  1.4%
Phe (F)   8	  2.9%
Pro (P)  10	  3.6%
Ser (S)  19	  6.8%
Thr (T)  24	  8.6%
Trp (W)   0	  0.0%
Tyr (Y)   6	  2.1%
Val (V)  23	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1276
Hydrogen    H	      2078
Nitrogen    N	       368
Oxygen      O	       404
Sulfur      S	         7

Formula: C1276H2078N368O404S7
Total number of atoms: 4133

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9065
Abs 0.1% (=1 g/l)   0.310, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.306, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.20
This classifies the protein as stable.



Aliphatic index: 92.39

Grand average of hydropathicity (GRAVY): 0.016