ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAQRHVSVGC FSTFSEAEAK SSEEWKTSTK LEALGSMEGE LAHLLSTCKP ETLNHNKTEL 

        70         80         90        100        110        120 
SGFRNLFARF LRAKPTVDWS KIEPLPEGAI RPYKSLGHVV DKELIASQLR KLVVVKLNGG 

       130        140        150        160        170        180 
LGTSMGCKGP KSVISVRNDL TFLDLTMQQI QTLNKTYGVD VPLVLMNSFN TNEDTQKVLK 

       190        200        210        220        230        240 
KYANVKVSVH TFSQSQYPRI NRETLLPIVK SLEADDNECW YPPGHGNFYE AFHNSGLLDK 

       250        260        270        280        290        300 
FLADGKEYCF LSNIDNMGAT VDLSILNFVL NPSPDQTAPE FLMEVTDKTR ADVKGGTLIQ 

       310        320        330        340        350        360 
YEGRLMLLEI AQVPKDYVDE FKSISKFRIF NTNNLWAKLD AMRKIVVENK LEMEVIVNPK 

       370        380        390        400        410        420 
HLDRGLDVIQ LETAAGAAIK SFSGAVGVNV PRSRFLPVKK TSDLLLLMSN LYDIDNGSLT 

       430        440        450        460        470        480 
LSHQRSFPTT PLVKLGSSFD KVKDYLGRFN GIPDLLELDH LTVSGDVWFG KDVSLKGTVI 

       490        500        510 
IIANHGDRID IPPGSILENK IVSGNLRILE H 

References and documentation are available.

Number of amino acids: 511

Molecular weight: 56901.39

Theoretical pI: 6.66

Amino acid composition: 
Ala (A)  25	  4.9%
Arg (R)  20	  3.9%
Asn (N)  30	  5.9%
Asp (D)  31	  6.1%
Cys (C)   5	  1.0%
Gln (Q)  13	  2.5%
Glu (E)  30	  5.9%
Gly (G)  34	  6.7%
His (H)  12	  2.3%
Ile (I)  27	  5.3%
Leu (L)  63	 12.3%
Lys (K)  39	  7.6%
Met (M)  11	  2.2%
Phe (F)  23	  4.5%
Pro (P)  23	  4.5%
Ser (S)  40	  7.8%
Thr (T)  29	  5.7%
Trp (W)   5	  1.0%
Tyr (Y)  11	  2.2%
Val (V)  40	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2550
Hydrogen    H	      4064
Nitrogen    N	       682
Oxygen      O	       757
Sulfur      S	        16

Formula: C2550H4064N682O757S16
Total number of atoms: 8069

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44140
Abs 0.1% (=1 g/l)   0.776, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   0.771, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 22.21
This classifies the protein as stable.



Aliphatic index: 96.28

Grand average of hydropathicity (GRAVY): -0.185