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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAQSKSNPPQ VPSGWKAVFD DEYQTWYYVD LSTNSSQWEP PRGTTWPRPK GPPPGVNNEK

70 80 90 100 110 120
SSRQQADQAP PPYSSQSTPQ VQAGAQAQQP RYYQPQQPQY PQYPQQQRYY PQQAPMPAAA

130 140 150 160 170 180
PQQAYYGTAP STSKGSGHGG AMMGGLLGVG AGLLGGAMLE HAFDDHNYDG PDTVVVENNY

190 200 210
YGDDAGGSDG GFDDAGGFDG GFDDGFDGSD F


References and documentation are available.

Number of amino acids: 211 Molecular weight: 22655.27 Theoretical pI: 4.26
Amino acid composition: 
Ala (A) 19 9.0% Arg (R) 5 2.4% Asn (N) 7 3.3% Asp (D) 18 8.5% Cys (C) 0 0.0% Gln (Q) 25 11.8% Glu (E) 5 2.4% Gly (G) 29 13.7% His (H) 3 1.4% Ile (I) 0 0.0% Leu (L) 6 2.8% Lys (K) 5 2.4% Met (M) 5 2.4% Phe (F) 7 3.3% Pro (P) 25 11.8% Ser (S) 16 7.6% Thr (T) 8 3.8% Trp (W) 4 1.9% Tyr (Y) 15 7.1% Val (V) 9 4.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 23 Total number of positively charged residues (Arg + Lys): 10 Atomic composition: Carbon C 996 Hydrogen H 1433 Nitrogen N 273 Oxygen O 329 Sulfur S 5 Formula: C996H1433N273O329S5 Total number of atoms: 3036 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 44350 Abs 0.1% (=1 g/l) 1.958 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 55.12 This classifies the protein as unstable. Aliphatic index: 32.46 Grand average of hydropathicity (GRAVY): -1.011