ProtParam
User-provided sequence:
10 20 30 40 50 60
MAQTPAFNKP KVELHVHLDG AIKPETILYY GKKRGIDLPA DTVEGLRNII GMDKPLSLPD
70 80 90 100 110 120
FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVDPIPWNQA
130 140 150 160 170 180
EGDLTPDEVV DLVNQGLQEG EQAFGIKVRS ILCCMRHQPS WSPEVLELCK KYHQKTVVAM
190 200 210 220 230 240
DLAGDETIEG SSLFPGHVEA YEGAVKDGIH RTVHAGEVGS AEVVREAVDI LKTERVGHGY
250 260 270 280 290 300
HTIEDEALYN RLLKENMHFE VCPWSSYLTG AWNPKTTHAV VRFKDDQANY SLNSDDPLIF
310 320 330 340 350
KSTVDTDYQM VKKDMGFTEE EFKRLNINAA KSSFLPEDEK KELLERLYKE YQ
References and
documentation are available.
Number of amino acids: 352
Molecular weight: 39899.40
Theoretical pI: 5.32
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1787
Hydrogen H 2778
Nitrogen N 472
Oxygen O 536
Sulfur S 14
Formula: C
1787H
2778N
472O
536S
14
Total number of atoms: 5587
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46090
Abs 0.1% (=1 g/l) 1.155, assuming all pairs of Cys residues form cystines
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 1.149, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.21
This classifies the protein as stable.
Aliphatic index: 83.35
Grand average of hydropathicity (GRAVY): -0.426