ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAQVEDYSSL HRLRKNSEIL SNANKILVAN RGEIPIRIFR SAHELSMQTV AIYSHEDRLS 

        70         80         90        100        110        120 
MHRLKADEAY VIGARGQYSP VQAYLQIDEI INIALEHNVS MIHPGYGFLS ENSEFARKVE 

       130        140        150        160        170        180 
DSGMIWIGPP HNVIDAVGDK VSARNLAGKC NVPVVPGTDG PIDSVEQAQE FVDKYGYPVI 

       190        200        210        220        230        240 
IKAAFGGGGR GMRVVREGES IADAFQRATS EAKTAFGNGT CFIERFLDKP KHIEVQLLAD 

       250        260        270        280        290        300 
NYGNVIHLFE RDCSVQRRHQ KVVEIAPAKT LPVEVRDAIL TDAVKLAKAA NYRNAGTAEF 

       310        320        330        340        350        360 
LVDNQNRHYF IEINPRIQVE HTITEEVTGV DIVAAQIQIA AGASLQQLGL LQDKITTRGF 

       370        380        390        400        410        420 
AIQCRITTED PAKNFQPDTG KIEVYRSSGG NGVRLDGGNG FAGAIISPHY DSMLVKCSTS 

       430        440        450        460        470        480 
GSNYEIARRK MIRALVEFRI RGVKTNIPFL LALLTHPTFV SGDCWTTFID DTPSLFEMVQ 

       490        500        510        520        530        540 
SKNRAQKLLS YLADLCVNGS SIKGQIGLPK LTRDADIPVI HDINGWDIDI KNTPPPESFR 

       550        560        570        580        590        600 
QYLLDYGPEQ FANQIRAFDG CLIMDTTWRD AHQSLLATRV RTIDLLNIAP ATAHAFRYAF 

       610        620        630        640        650        660 
ALECWGGATF DVAMRFLHED PWDRLRKLRK AVPNIPFQML LRGANGVAYS SLPDNAIDHF 

       670        680        690        700        710        720 
VKQAKDAGVD IFRVFDALND LEQLKVGVDA VKKAGGVVEA TVCYSGDMLK PGKKYNLKYY 

       730        740        750        760        770        780 
LETVDKIMEM GTHLLGIKDM AGTLKPAAAK LLISSIRKKY PSVPIHVHTH DSAGTGVITY 

       790        800        810        820        830        840 
VACALAGADV VDCAVNSMSG LTSQPSMSAF IAALDNEINT GITEQNAREI DAYWSEMRLL 

       850        860        870        880        890        900 
YSCFEADLKG PDPEVYNHEI PGGQLTNLLF QAQQVGLGEK WLETKKAYEE ANMLLGDIVK 

       910        920        930        940        950        960 
VTPTSKVVGD LAQFMVSNKL SPKDVERLAS ELDFPDSVLD FFEGLMGTPY GGFPEPLRTN 

       970        980        990       1000       1010       1020 
ILAGKRRKLT RRPGLELEPF DLKKIKEELQ SRFGNSITEC DVASYNMYPK VFESFKKIQE 

      1030       1040       1050       1060       1070       1080 
KYGDLSVLPT RFFLAPPKLN EEISVEIEQG KTFVIKVMAI GDLSPQTGTR EVYFEFNGEM 

      1090       1100       1110       1120       1130       1140 
RKVTVEDKLA AVETVTRPKA DAHNPNEVGA PMAGVVIEVR VHPGVEVKKG DPLCVLSAMK 

      1150       1160       1170 
MEMVISSPVS GRVGEVIVHE NDSVDAGDLI CKITK 

References and documentation are available.

Number of amino acids: 1175

Molecular weight: 129810.64

Theoretical pI: 5.95

Amino acid composition: 
Ala (A) 102	  8.7%
Arg (R)  61	  5.2%
Asn (N)  50	  4.3%
Asp (D)  73	  6.2%
Cys (C)  16	  1.4%
Gln (Q)  41	  3.5%
Glu (E)  76	  6.5%
Gly (G)  87	  7.4%
His (H)  27	  2.3%
Ile (I)  81	  6.9%
Leu (L)  97	  8.3%
Lys (K)  71	  6.0%
Met (M)  29	  2.5%
Phe (F)  48	  4.1%
Pro (P)  57	  4.9%
Ser (S)  65	  5.5%
Thr (T)  57	  4.9%
Trp (W)   8	  0.7%
Tyr (Y)  34	  2.9%
Val (V)  95	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 149
Total number of positively charged residues (Arg + Lys): 132

Atomic composition:

Carbon      C	      5781
Hydrogen    H	      9166
Nitrogen    N	      1582
Oxygen      O	      1721
Sulfur      S	        45

Formula: C5781H9166N1582O1721S45
Total number of atoms: 18295

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    95660
Abs 0.1% (=1 g/l)   0.737, assuming all pairs of Cys residues form cystines


Ext. coefficient    94660
Abs 0.1% (=1 g/l)   0.729, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.99
This classifies the protein as stable.



Aliphatic index: 91.21

Grand average of hydropathicity (GRAVY): -0.172