ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARDMNVVEI LDDEEEDEKE CEDWCFICKD GGNLMLCDFK DCPKVYHESC VEKDSSASKN 

        70         80         90        100        110        120 
GDSYICMWHS CYLCKKTPKL CCLCCSHAVC EGCVTHAEFI QLKGDKGLCN QCQEYVFALE 

       130        140        150        160        170        180 
EIQEYDAAGD KLDLTDRNTF ECLFLEYWEI AKKQEGLTFD DVRKVCASKP QKKGVKSKYK 

       190        200        210        220        230        240 
DDPKFSLGDV HTSKSQKKGD KLKNKDDPKF ALGDAHTSKS GKKGVKLKNK DDPKFLVSDH 

       250        260        270        280        290        300 
AVEDAVDYKK VGKNKRMEFI RWGSKPLIDF LTSIGEDTRE AMSQHSVESV IRRYIREKNL 

       310        320        330        340        350        360 
LDREKKKKVH CDEKLYSIFR KKSINQKRIY TLLNTHLKEN LDQVEYFTPL ELGFIEKNEK 

       370        380        390        400        410        420 
RFSEKNDKVM MPCKKQKTES SDDEICEKEV QPEMRATGFA TINADNLKLV YLRKSLVLEL 

       430        440        450        460        470        480 
LKQNDSFVDK VVGSFVKVKN GPRDFMAYQI LQVTGIKNAD DQSEGVLLHV SGMASGVSIS 

       490        500        510        520        530        540 
KLDDSDIREE EIKDLKQKVM NGLLRQTTVV EMEQKAKALH YDITKHRELL EKPSEQERLL 

       550        560        570 
KEIPRIIEDF IEIKQEPCVG GLSHEAVINI D 

References and documentation are available.

Number of amino acids: 571

Molecular weight: 65601.15

Theoretical pI: 6.14

Amino acid composition: 
Ala (A)  23	  4.0%
Arg (R)  21	  3.7%
Asn (N)  21	  3.7%
Asp (D)  50	  8.8%
Cys (C)  23	  4.0%
Gln (Q)  21	  3.7%
Glu (E)  54	  9.5%
Gly (G)  28	  4.9%
His (H)  14	  2.5%
Ile (I)  30	  5.3%
Leu (L)  50	  8.8%
Lys (K)  76	 13.3%
Met (M)  13	  2.3%
Phe (F)  21	  3.7%
Pro (P)  14	  2.5%
Ser (S)  36	  6.3%
Thr (T)  19	  3.3%
Trp (W)   4	  0.7%
Tyr (Y)  15	  2.6%
Val (V)  38	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      2876
Hydrogen    H	      4614
Nitrogen    N	       784
Oxygen      O	       892
Sulfur      S	        36

Formula: C2876H4614N784O892S36
Total number of atoms: 9202

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45725
Abs 0.1% (=1 g/l)   0.697, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   0.676, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.53
This classifies the protein as stable.



Aliphatic index: 77.97

Grand average of hydropathicity (GRAVY): -0.663