ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE 

        70         80         90        100        110        120 
VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS 

       130        140        150        160        170        180 
YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV KIDLASGKIT DFIKFDAGKL 

       190        200        210        220        230        240 
VYVTGGRNLG RIGTIVHKER HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK 

       250        260 
GKGIKLSIAE ERDRRRAQQG L 

References and documentation are available.

Number of amino acids: 261

Molecular weight: 29410.19

Theoretical pI: 10.09

Amino acid composition: 
Ala (A)  15	  5.7%
Arg (R)  22	  8.4%
Asn (N)  10	  3.8%
Asp (D)  18	  6.9%
Cys (C)   1	  0.4%
Gln (Q)   5	  1.9%
Glu (E)   9	  3.4%
Gly (G)  24	  9.2%
His (H)  10	  3.8%
Ile (I)  16	  6.1%
Leu (L)  25	  9.6%
Lys (K)  27	 10.3%
Met (M)   3	  1.1%
Phe (F)   9	  3.4%
Pro (P)  12	  4.6%
Ser (S)   8	  3.1%
Thr (T)  14	  5.4%
Trp (W)   1	  0.4%
Tyr (Y)   9	  3.4%
Val (V)  23	  8.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      1321
Hydrogen    H	      2144
Nitrogen    N	       390
Oxygen      O	       362
Sulfur      S	         4

Formula: C1321H2144N390O362S4
Total number of atoms: 4221

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.643, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.643, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.73
This classifies the protein as stable.



Aliphatic index: 92.57

Grand average of hydropathicity (GRAVY): -0.448