ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARGPNKVLL VPYLVDYVLP FLIGLVKHKD VCALASLDPV RLYAGLGYMP IRKNHVAAIV 

        70         80         90        100        110        120 
DYVCSSNVAL SSNQTKLQLA FIQHFLSAEL LQLTEEEKNK ILEFVVSNLY NEQFVEVRVR 

       130        140        150        160        170        180 
AASILSDIVH NWKEEQPLLS LIERFAKGLD VNKYTSKERQ KLSKTDIKIH GNVLGLGAII 

       190        200        210        220        230        240 
SAFPYVFPLP PWIPKQLSNL SSWARTSGMT GQAAKNTISE FKKVRADTWK FDRASFNTEE 

       250 
LEDLEGVLWR SYYA 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 28760.32

Theoretical pI: 8.90

Amino acid composition: 
Ala (A)  20	  7.9%
Arg (R)  11	  4.3%
Asn (N)  13	  5.1%
Asp (D)  10	  3.9%
Cys (C)   2	  0.8%
Gln (Q)   9	  3.5%
Glu (E)  16	  6.3%
Gly (G)  11	  4.3%
His (H)   5	  2.0%
Ile (I)  14	  5.5%
Leu (L)  33	 13.0%
Lys (K)  19	  7.5%
Met (M)   3	  1.2%
Phe (F)  11	  4.3%
Pro (P)  11	  4.3%
Ser (S)  20	  7.9%
Thr (T)   9	  3.5%
Trp (W)   5	  2.0%
Tyr (Y)  10	  3.9%
Val (V)  22	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1317
Hydrogen    H	      2073
Nitrogen    N	       343
Oxygen      O	       368
Sulfur      S	         5

Formula: C1317H2073N343O368S5
Total number of atoms: 4106

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42525
Abs 0.1% (=1 g/l)   1.479, assuming all pairs of Cys residues form cystines


Ext. coefficient    42400
Abs 0.1% (=1 g/l)   1.474, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.37
This classifies the protein as stable.



Aliphatic index: 105.16

Grand average of hydropathicity (GRAVY): -0.044