ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARKFFVGGN WKCNGTAEEV KKIVNTLNEA QVPSQDVVEV VVSPPYVFLP LVKSTLRSDF 

        70         80         90        100        110        120 
FVAAQNCWVK KGGAFTGEVS AEMLVNLDIP WVILGHSERR AILNESSEFV GDKVAYALAQ 

       130        140        150        160        170        180 
GLKVIACVGE TLEEREAGST MDVVAAQTKA IADRVTNWSN VVIAYEPVWA IGTGKVASPA 

       190        200        210        220        230        240 
QAQEVHDELR KWLAKNVSAD VAATTRIIYG GSVNGGNCKE LGGQADVDGF LVGGASLKPE 

       250 
FIDIIKAAEV KKSA 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 27169.08

Theoretical pI: 5.39

Amino acid composition: 
Ala (A)  31	 12.2%
Arg (R)   8	  3.1%
Asn (N)  12	  4.7%
Asp (D)  11	  4.3%
Cys (C)   4	  1.6%
Gln (Q)   8	  3.1%
Glu (E)  19	  7.5%
Gly (G)  22	  8.7%
His (H)   2	  0.8%
Ile (I)  13	  5.1%
Leu (L)  16	  6.3%
Lys (K)  18	  7.1%
Met (M)   3	  1.2%
Phe (F)   9	  3.5%
Pro (P)   8	  3.1%
Ser (S)  15	  5.9%
Thr (T)  11	  4.3%
Trp (W)   6	  2.4%
Tyr (Y)   4	  1.6%
Val (V)  34	 13.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1215
Hydrogen    H	      1930
Nitrogen    N	       326
Oxygen      O	       365
Sulfur      S	         7

Formula: C1215H1930N326O365S7
Total number of atoms: 3843

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39210
Abs 0.1% (=1 g/l)   1.443, assuming all pairs of Cys residues form cystines


Ext. coefficient    38960
Abs 0.1% (=1 g/l)   1.434, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.37
This classifies the protein as stable.



Aliphatic index: 95.55

Grand average of hydropathicity (GRAVY): 0.076