ProtParam
User-provided sequence:
10 20 30 40 50 60
MARKGSSIRL SSSRISTLLL FMFATFASFY VAGRLWQESQ TRVHLINELD RVTGQGKSAI
70 80 90 100 110 120
SVDDTLKIIA CREQKKTLAA LEMELSSARQ EGFVSKSPKL ADGTETKKRP LVVIGIMTSL
130 140 150 160 170 180
GNKKKRDAVR QAWMGTGASL KKLESEKGVI ARFVIGRSAN KGDSMDKSID TENSQTDDFI
190 200 210 220 230 240
ILDDVVEAPE EASKKVKLFF AYAADRWDAQ FYAKAIDNIY VNIDALGTTL AAHLENPRAY
250 260 270 280 290 300
IGCMKSGEVF SEPNHKWYEP EWWKFGDKKA YFRHAYGEMY VITHALARFV SINRDILHSY
310 320 330
AHDDVSTGSW FVGLDVKHVD EGKFCCSAWS SEAICAGV
References and
documentation are available.
Number of amino acids: 338
Molecular weight: 37768.07
Theoretical pI: 8.43
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1686
Hydrogen H 2639
Nitrogen N 461
Oxygen O 499
Sulfur S 13
Formula: C
1686H
2639N
461O
499S
13
Total number of atoms: 5298
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59150
Abs 0.1% (=1 g/l) 1.566, assuming all pairs of Cys residues form cystines
Ext. coefficient 58900
Abs 0.1% (=1 g/l) 1.560, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.09
This classifies the protein as stable.
Aliphatic index: 81.15
Grand average of hydropathicity (GRAVY): -0.257