ProtParam
User-provided sequence:
10 20 30 40 50 60
MARKHTFPSL KRAEILECID GLGIPFTAKE LDQPTSKAVI PLYEEFLDLF MGLTRQNLEE
70 80 90 100 110 120
KVNSLQDSVE NFEIIHESLR FTVFYQILSQ FMQNICFHDF TIQDLLKPDR NRLQLILSAV
130 140 150 160 170 180
INFAKLREER LQQFDDDIQK RESLLETYTL LDAQRKDLEE KVLLSQDRKL ESEAIIKQNE
190 200 210 220 230 240
ERNEEMFQSL IEDKRLCSQV RTEYDRIRME ASELKIRYHN VDSLMASTLE EIEKLQSSIV
250 260 270 280 290 300
HSPEKLKGKI ADTSLRIQND RSQQVELDKK SKILHTKLNS LQLIEGDLNA CLKVLEECLV
310 320 330 340 350 360
ELDKLEHATV LLSTNQELCD QIEINKKKLE FRKEQLLKQL SNAQEKLEHE QHSRNQKLEA
370 380 390 400 410 420
AKQRMDNIRE EYKVITQERN KKIQETEKKN AMIEMTEQKI AGMREELESQ ISSITMEFEK
430 440
LKSHVELYIA ELLRNLRSSN S
References and
documentation are available.
Number of amino acids: 441
Molecular weight: 51911.39
Theoretical pI: 5.44
Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 67
Atomic composition:
Carbon C 2270
Hydrogen H 3724
Nitrogen N 636
Oxygen O 715
Sulfur S 17
Formula: C
2270H
3724N
636O
715S
17
Total number of atoms: 7362
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10805
Abs 0.1% (=1 g/l) 0.208, assuming all pairs of Cys residues form cystines
Ext. coefficient 10430
Abs 0.1% (=1 g/l) 0.201, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.19
This classifies the protein as unstable.
Aliphatic index: 97.30
Grand average of hydropathicity (GRAVY): -0.648