ProtParam
User-provided sequence:
10 20 30 40 50 60
MARKKIREYD SKRLLKEHLK RLANIDLQIR SAQVTESTDF TELTNQESWL SSTKLVVKPD
70 80 90 100 110 120
MLFGKRGKSG LVALKLDLAE VADFVKARLG TEVEMEGCKA PITTFIVEPF VPHDQEYYLS
130 140 150 160 170 180
IVSDRLGCTI SFSECGGIEI EENWDKVKTI FLPAEKSMTL EVCAPLIATL PLEVRAKIGN
190 200 210 220 230 240
FIMGAFAVFQ DLDFSFMEMN PFTLVDGEPF PLDMRGELDD TAAFKNFNKW GDIEFPLPFG
250 260 270 280 290 300
RVLSSTENFI HGLDEKTSAS LKFTVLNPKG RIWTMVAGGG ASVIYADTVG DLGYASELGN
310 320 330 340 350 360
YAEYSGAPNE EEVLQYARVV IDCATTDPDG RKRALLIGGG IANFTDVAAT FNGIIRALRE
370 380 390 400 410 420
KETRLKASRM HIYVRRGGPN YQTGLARMRA LGEELGVPLE VYGPEATMTG ICKRAIDCIM
LPDA
References and
documentation are available.
Number of amino acids: 424
Molecular weight: 46945.98
Theoretical pI: 5.16
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 2098
Hydrogen H 3320
Nitrogen N 554
Oxygen O 625
Sulfur S 20
Formula: C
2098H
3320N
554O
625S
20
Total number of atoms: 6617
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38765
Abs 0.1% (=1 g/l) 0.826, assuming all pairs of Cys residues form cystines
Ext. coefficient 38390
Abs 0.1% (=1 g/l) 0.818, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.59
This classifies the protein as stable.
Aliphatic index: 89.50
Grand average of hydropathicity (GRAVY): -0.097