ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARKRAAGGE PRGRELRSQK SKAKSKARRE EEEEDAFEDE KPPKKSLLSK VSQGKRKRGC 

        70         80         90        100        110        120 
SHPGGSADGP AKKKVAKVTV KSENLKVIKD EALSDGDDLR DFPSDLKKAH HLKRGATMNE 

       130        140        150        160        170        180 
DSNEEEEESE NDWEEVEELS EPVLGDVRES TAFSRSLLPV KPVEIEIETP EQAKTRERSE 

       190        200        210        220        230        240 
KIKLEFETYL RRAMKRFNKG VHEDTHKVHL LCLLANGFYR NNICSQPDLH AIGLSIIPAR 

       250        260        270        280        290        300 
FTRVLPRDVD TYYLSNLVKW FIGTFTVNAE LSASEQDNLQ TTLERRFAIY SARDDEELVH 

       310        320        330        340        350        360 
IFLLILRALQ LLTRLVLSLQ PIPLKSATAK GKKPSKERLT ADPGGSSETS SQVLENHTKP 

       370        380        390        400        410        420 
KTSKGTKQEE TFAKGTCRPS AKGKRNKGGR KKRSKPSSSE EDEGPGDKQE KATQRRPHGR 

       430        440        450        460        470        480 
ERRVASRVSY KEESGSDEAG SGSDFELSSG EASDPSDEDS EPGPPKQRKA PAPQRTKAGS 

       490        500        510        520        530        540 
KSASRTHRGS HRKDPSLPAA SSSSSSSKRG KKMCSDGEKA EKRSIAGIDQ WLEVFCEQEE 

       550        560        570        580        590        600 
KWVCVDCVHG VVGQPLTCYK YATKPMTYVV GIDSDGWVRD VTQRYDPVWM TVTRKCRVDA 

       610        620        630        640        650        660 
EWWAETLRPY QSPFMDREKK EDLEFQAKHM DQPLPTAIGL YKNHPLYALK RHLLKYEAIY 

       670        680        690        700        710        720 
PETAAILGYC RGEAVYSRDC VHTLHSRDTW LKKARVVRLG EVPYKMVKGF SNRARKARLA 

       730        740        750        760        770        780 
EPQLREENDL GLFGYWQTEE YQPPVAVDGK VPRNEFGNVY LFLPSMMPIG CVQLNLPNLH 

       790        800        810        820        830        840 
RVARKLDIDC VQAITGFDFH GGYSHPVTDG YIVCEEFKDV LLTAWENEQA VIERKEKEKK 

       850        860        870        880        890        900 
EKRALGNWKL LAKGLLIRER LKRRYGPKSE AAAPHTDAGG GLSSDEEEGT SSQAEAARIL 

       910        920        930        940 
AASWPQNRED EEKQKLKGGP KKTKREKKAA ASHLFPFEKL 

References and documentation are available.

Number of amino acids: 940

Molecular weight: 105952.73

Theoretical pI: 9.05

Amino acid composition: 
Ala (A)  73	  7.8%
Arg (R)  73	  7.8%
Asn (N)  23	  2.4%
Asp (D)  51	  5.4%
Cys (C)  15	  1.6%
Gln (Q)  30	  3.2%
Glu (E)  95	 10.1%
Gly (G)  63	  6.7%
His (H)  23	  2.4%
Ile (I)  26	  2.8%
Leu (L)  78	  8.3%
Lys (K)  94	 10.0%
Met (M)  11	  1.2%
Phe (F)  25	  2.7%
Pro (P)  53	  5.6%
Ser (S)  76	  8.1%
Thr (T)  43	  4.6%
Trp (W)  13	  1.4%
Tyr (Y)  24	  2.6%
Val (V)  51	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 146
Total number of positively charged residues (Arg + Lys): 167

Atomic composition:

Carbon      C	      4634
Hydrogen    H	      7419
Nitrogen    N	      1365
Oxygen      O	      1429
Sulfur      S	        26

Formula: C4634H7419N1365O1429S26
Total number of atoms: 14873

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   108135
Abs 0.1% (=1 g/l)   1.021, assuming all pairs of Cys residues form cystines


Ext. coefficient   107260
Abs 0.1% (=1 g/l)   1.012, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.22
This classifies the protein as unstable.



Aliphatic index: 66.65

Grand average of hydropathicity (GRAVY): -0.874