ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL GQPNTTSNYG 

        70         80         90        100        110        120 
SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL SSANTNTFYS VKSNVPNIES 

       130        140        150        160        170        180 
KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA ENFISGMSAH DYKLMNISKE SFESKYKLSS 

       190        200        210        220        230        240 
NTMYNKSMNV YCDGSSFGNG TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS 

       250        260        270        280        290        300 
EALKKIWEKL TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF 

       310        320        330        340 
VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR 

References and documentation are available.

Number of amino acids: 348

Molecular weight: 39431.05

Theoretical pI: 9.35

Amino acid composition: 
Ala (A)  21	  6.0%
Arg (R)  14	  4.0%
Asn (N)  32	  9.2%
Asp (D)  12	  3.4%
Cys (C)   5	  1.4%
Gln (Q)  12	  3.4%
Glu (E)  24	  6.9%
Gly (G)  25	  7.2%
His (H)   7	  2.0%
Ile (I)  13	  3.7%
Leu (L)  19	  5.5%
Lys (K)  37	 10.6%
Met (M)   7	  2.0%
Phe (F)  15	  4.3%
Pro (P)   9	  2.6%
Ser (S)  37	 10.6%
Thr (T)  18	  5.2%
Trp (W)   4	  1.1%
Tyr (Y)  21	  6.0%
Val (V)  16	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      1735
Hydrogen    H	      2681
Nitrogen    N	       489
Oxygen      O	       541
Sulfur      S	        12

Formula: C1735H2681N489O541S12
Total number of atoms: 5458

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53540
Abs 0.1% (=1 g/l)   1.358, assuming all pairs of Cys residues form cystines


Ext. coefficient    53290
Abs 0.1% (=1 g/l)   1.351, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.43
This classifies the protein as stable.



Aliphatic index: 55.23

Grand average of hydropathicity (GRAVY): -0.873