ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL 

        70         80         90        100        110        120 
SSEALEAARI CANKYMTTVS GRDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG 

       130        140        150        160        170        180 
KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP GQQKIILSKK WGFTNLDRPE 

       190        200        210        220 
YLKKREAGEV KDDGAFVKFL SKKGSLENNI REFPEYFAAQ A 

References and documentation are available.

Number of amino acids: 221

Molecular weight: 25361.34

Theoretical pI: 10.03

Amino acid composition: 
Ala (A)  20	  9.0%
Arg (R)  22	 10.0%
Asn (N)   9	  4.1%
Asp (D)  11	  5.0%
Cys (C)   4	  1.8%
Gln (Q)   8	  3.6%
Glu (E)  12	  5.4%
Gly (G)  14	  6.3%
His (H)   5	  2.3%
Ile (I)  10	  4.5%
Leu (L)  17	  7.7%
Lys (K)  22	 10.0%
Met (M)   4	  1.8%
Phe (F)  10	  4.5%
Pro (P)  10	  4.5%
Ser (S)  12	  5.4%
Thr (T)   5	  2.3%
Trp (W)   2	  0.9%
Tyr (Y)   9	  4.1%
Val (V)  15	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1130
Hydrogen    H	      1808
Nitrogen    N	       338
Oxygen      O	       311
Sulfur      S	         8

Formula: C1130H1808N338O311S8
Total number of atoms: 3595

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   0.972, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.962, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.78
This classifies the protein as stable.



Aliphatic index: 76.38

Grand average of hydropathicity (GRAVY): -0.610