ProtParam
User-provided sequence:
10 20 30 40 50 60
MARRPYDMLF KLLLIGDSGV GKTCILYRFS DDAFNTTFIS TIGIDFKIKT IELKGKKIKL
70 80 90 100 110 120
QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNAKSFDNI AKWLRNIDEH ASEDVVKMIL
130 140 150 160 170 180
GNKCDMSDRR VVSRERGEKI AQDHGISFHE TSAKLNVHVD TAFYDLAEAI LAKMPDSTDE
190 200
QSRDTVNPVQ PQRQSSSGGC C
References and
documentation are available.
Number of amino acids: 201
Molecular weight: 22711.91
Theoretical pI: 6.96
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 998
Hydrogen H 1586
Nitrogen N 278
Oxygen O 305
Sulfur S 11
Formula: C
998H
1586N
278O
305S
11
Total number of atoms: 3178
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20190
Abs 0.1% (=1 g/l) 0.889, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.878, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.36
This classifies the protein as unstable.
Aliphatic index: 81.04
Grand average of hydropathicity (GRAVY): -0.352