ProtParam
User-provided sequence:
10 20 30 40 50 60
MARRSVSIFN RLLANPPSPF TSLSRSITYM PRPGDGAPRT VTLIPGDGIG PLVTGAVEQV
70 80 90 100 110 120
MEAMHAPVHF ERYEVLGNMR KVPEEVIESV KRNKVCLKGG LATPVGGGVS SLNMQLRKEL
130 140 150 160 170 180
DIFASLVNCI NVPGLVTRHE NVDIVVIREN TEGEYSGLEH EVVPGVVESL KVITKFCSER
190 200 210 220 230 240
IARYAFEYAY LNNRKKVTAV HKANIMKLAD GLFLESCREV AKHYSGITYN EIIVDNCCMQ
250 260 270 280 290 300
LVAKPEQFDV MVTPNLYGNL IANTAAGIAG GTGVMPGGNV GAEHAIFEQG ASAGNVGNDK
310 320 330 340 350 360
MVEQKKANPV ALLLSSAMML RHLRFPTFAD RLETAVKQVI KEGKYRTKDL GGDCTTQEVV
DAVIAALE
References and
documentation are available.
Number of amino acids: 368
Molecular weight: 39957.17
Theoretical pI: 7.08
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1760
Hydrogen H 2849
Nitrogen N 493
Oxygen O 525
Sulfur S 20
Formula: C
1760H
2849N
493O
525S
20
Total number of atoms: 5647
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15275
Abs 0.1% (=1 g/l) 0.382, assuming all pairs of Cys residues form cystines
Ext. coefficient 14900
Abs 0.1% (=1 g/l) 0.373, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.40
This classifies the protein as stable.
Aliphatic index: 93.99
Grand average of hydropathicity (GRAVY): -0.029