ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MARSRKNSLV RDIASHPTIP ESQTIVGLLD DSYLFDKLKK LSLAVENSDS LQRTDVSEGC 

        70         80         90        100        110        120 
SEVNGSEATT SADVKKTNKY LYYTTYLDQL NIKIDEYKVV LDQTRQVNDQ LDSSIKKFRK 

       130        140        150        160        170        180 
ISQDTGAFIE ETKTIYEKQS KLSNLTESIP KALHYFEVLD PIMRRLNHAT SPAIVKKSSF 

       190        200        210        220        230        240 
TTMLATIDES LRFLDENSDL KDAAAYRIKF KQCLIRACEL ISHFLTNLLK QTNQEILDKT 

       250        260        270        280        290        300 
KNKNSLTGLP STTRDAFLYS KFYTIADTFK IQVSEIVKRS NEKAYNKYHD ELNSILYECF 

       310        320        330        340        350        360 
NHYFQTRLRL LTPVIWSHID EIVVKDKDQG LVKFIQDGKV YFQQLCADEY KLFVEFFPEK 

       370        380        390        400        410        420 
ECRFKINQWF LQLCEPLYDS IRVRVLKETD ICTLCDSVTL FAPYYEFEEG SEEYVKQFTD 

       430        440        450        460        470        480 
IQYDKLFEPI VQKVQARLIL RVQIYVQQNI LSYRPTRDVF MISNRRRKSK TSLQGGNEDA 

       490        500        510        520        530        540 
TTSDDNPDPL LESYLSSFKN RSILPISPND ADDKSIDSEE STDKISQLQT YYPPLLKTLA 

       550        560        570        580        590        600 
LLSKIYEMIN SVVFDDLAHH VVHDCIVSLR NAYDMVIKSS AGKSDFNNLD ISLAYLKNLL 

       610        620        630        640        650        660 
MLRDSIQNFN IQYTVNETYL DFSGVEGFFK SLKENGRNVL KKTKSSSILT LARELVPKVV 

       670        680        690        700        710        720 
NNMVDARTEL ISELRNVIKD FTESTSLELI DDTLDINSDE DLLSKNVKLR ENIKARLPRI 

       730        740        750        760        770        780 
YEQILNYIDD QEIVTNLLDA VQELITQSYS KYYETITELA ENGKFAKDQV ADVMYLDVFT 

       790        800 
DFFAKEVADL LRNGDIDTIT K 

References and documentation are available.

Number of amino acids: 801

Molecular weight: 92544.18

Theoretical pI: 5.28

Amino acid composition: 
Ala (A)  36	  4.5%
Arg (R)  36	  4.5%
Asn (N)  44	  5.5%
Asp (D)  67	  8.4%
Cys (C)  10	  1.2%
Gln (Q)  36	  4.5%
Glu (E)  54	  6.7%
Gly (G)  16	  2.0%
His (H)  10	  1.2%
Ile (I)  59	  7.4%
Leu (L)  91	 11.4%
Lys (K)  66	  8.2%
Met (M)   9	  1.1%
Phe (F)  37	  4.6%
Pro (P)  20	  2.5%
Ser (S)  68	  8.5%
Thr (T)  53	  6.6%
Trp (W)   2	  0.2%
Tyr (Y)  38	  4.7%
Val (V)  49	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 121
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      4139
Hydrogen    H	      6557
Nitrogen    N	      1077
Oxygen      O	      1283
Sulfur      S	        19

Formula: C4139H6557N1077O1283S19
Total number of atoms: 13075

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68245
Abs 0.1% (=1 g/l)   0.737, assuming all pairs of Cys residues form cystines


Ext. coefficient    67620
Abs 0.1% (=1 g/l)   0.731, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.03
This classifies the protein as stable.



Aliphatic index: 95.27

Grand average of hydropathicity (GRAVY): -0.385