ProtParam
User-provided sequence:
10 20 30 40 50 60
MARTATIKRD TNETKIQIAL SLDGGFLAVE ESIFKKRKID DDDEHAKQAT SSQVINVQSG
70 80 90 100 110 120
IGFLDHMLHA LAKHAGWSLI VECIGDLHID DHHTAEDVGI TLGIAFKQAL GQVKGVKRFG
130 140 150 160 170 180
SGFAPLDEAL SRAVVDLSNR PFAVVELGLK REKIGDLSCE MIPHVLESFA QGAHITTHVD
190 200 210 220
CLRGFNDHHR AESAFKALAV AIREAISSNG TNDVPSTKGV LF
References and
documentation are available.
Number of amino acids: 222
Molecular weight: 24057.36
Theoretical pI: 6.04
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1061
Hydrogen H 1698
Nitrogen N 304
Oxygen O 322
Sulfur S 6
Formula: C
1061H
1698N
304O
322S
6
Total number of atoms: 3391
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 5625
Abs 0.1% (=1 g/l) 0.234, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.229, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.54
This classifies the protein as stable.
Aliphatic index: 96.71
Grand average of hydropathicity (GRAVY): -0.071