ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASADDYFSD FEDDELDKLY EKAINKSVKE TITRRAVPVQ KDLHDNVLPG QKTVYEEIQR 

        70         80         90        100        110        120 
DVSFGPTHHE LDYDALSFYV YPTNYEVRDY QYTIVHKSLF QNTLCAIPTG MGKTFIASTV 

       130        140        150        160        170        180 
MLNYFRWTKK AKIIFTAPTR PLVAQQIKAC LGITGIPSDQ TAILLDKSRK NREEIWANKR 

       190        200        210        220        230        240 
VFFATPQVVE NDLKRGVLDP KDIVCLVIDE AHRATGSSAY TNVVKFIDRF NSSYRLLALT 

       250        260        270        280        290        300 
ATPASDLEGV QEVVNNLDIS KIEIRTEESM DIVKYMKKRK KEKIEVPLLL EIEDIIEQLG 

       310        320        330        340        350        360 
MAVKPVLQQA IELGIYEECD PSQINAFKAM QQSQKIIANP TIPEGIKWRN FFILQLLNNV 

       370        380        390        400        410        420 
GQMLKRLKIY GIRTFFNYFQ NKCTEFTTKY NLKKSTNKIA AEFYYHPILK NIKNQCENYL 

       430        440        450        460        470        480 
SDPKFVGHGK LQCVRDELMD FFQKRGSDSR VIIFTELRES ALEIVKFIDS VADDQIRPHI 

       490        500        510        520        530        540 
FIGQARAKEG FDEVKYTRKH APKGRKKVER LHRQEQEKFL EAERTKRAAN DKLERSARRT 

       550        560        570        580        590        600 
GSSEEAQISG MNQKMQKEVI HNFKKGEYNV LVCTSIGEEG LDIGEVDLII CYDTTSSPIK 

       610        620        630        640        650        660 
NIQRMGRTGR KRDGKIVLLF SSNESYKFER AMEDYSTLQA LISKQCIDYK KSDRIIPEDI 

       670        680        690        700        710        720 
IPECHETLIT INDENEIINE MEDVDEVIRY ATQCMMGKKV KPKKAITKKK RVQENKKPKK 

       730        740        750        760        770        780 
FFMPDNVETS IVSASTLINK FLVNESGGKQ LVTSNENPSK KRKIFKALDN LENDSTEEAS 

       790        800        810        820        830        840 
SSLETEDEEV SDDNNVFIAE GQNGCQKDLE TAIIRTGESL TTLKPLHNFE RPNMALFVND 

       850        860        870        880        890        900 
CGLPTKIEKN VKDIRGNQHN LEKEKSCTVD KNNMVLSLDD WNFFRNRYIP EGVSFDVEPN 

       910        920        930        940        950        960 
FVQYTKGVKV PHCHKVSKII TLFNDESNDN KKRTIDMNYT KCLARGMLRD EKKFVKVNDK 

       970        980        990 
SQVDNNSVNH DSSQSFTLSN AELDDILGSD SDF 

References and documentation are available.

Number of amino acids: 993

Molecular weight: 114057.99

Theoretical pI: 7.00

Amino acid composition: 
Ala (A)  49	  4.9%
Arg (R)  50	  5.0%
Asn (N)  64	  6.4%
Asp (D)  68	  6.8%
Cys (C)  17	  1.7%
Gln (Q)  42	  4.2%
Glu (E)  82	  8.3%
Gly (G)  41	  4.1%
His (H)  17	  1.7%
Ile (I)  78	  7.9%
Leu (L)  71	  7.2%
Lys (K)  99	 10.0%
Met (M)  21	  2.1%
Phe (F)  46	  4.6%
Pro (P)  33	  3.3%
Ser (S)  62	  6.2%
Thr (T)  56	  5.6%
Trp (W)   4	  0.4%
Tyr (Y)  30	  3.0%
Val (V)  63	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 150
Total number of positively charged residues (Arg + Lys): 149

Atomic composition:

Carbon      C	      5041
Hydrogen    H	      8048
Nitrogen    N	      1386
Oxygen      O	      1548
Sulfur      S	        38

Formula: C5041H8048N1386O1548S38
Total number of atoms: 16061

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67700
Abs 0.1% (=1 g/l)   0.594, assuming all pairs of Cys residues form cystines


Ext. coefficient    66700
Abs 0.1% (=1 g/l)   0.585, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.41
This classifies the protein as stable.



Aliphatic index: 81.85

Grand average of hydropathicity (GRAVY): -0.581