ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASAMVTSLR LRPNFSAATS PSSSSSAGNV KYRPAVILPG LGNNTGDYKK LEVTLGEYGV 

        70         80         90        100        110        120 
PAVVAAVSRL DWFRNAAGLV DPAYWRGTLR PRPVLDWYLN RIDDAVREAN ELAQGQGLCL 

       130        140        150        160        170        180 
IGHSAGGWLA RVYMEEYGNS DISLLLTLGT PHLPPPRGLP GVIDQTRGLL YYVEENCAKA 

       190        200        210        220        230        240 
VYTPELKYVC IAGRYIRGAR LVDNADADID SDVTVGIDSG EGISELAIAS NKKSGTFRAR 

       250        260        270        280        290        300 
FVGQGYKQVC GRADVWGDGV VPEVSAHLEG ALNVSFDGVY HSPVGSDDET RPWYGSPVIV 


KDWIHHLLE 

References and documentation are available.

Number of amino acids: 309

Molecular weight: 33407.72

Theoretical pI: 5.56

Amino acid composition: 
Ala (A)  29	  9.4%
Arg (R)  20	  6.5%
Asn (N)  12	  3.9%
Asp (D)  20	  6.5%
Cys (C)   4	  1.3%
Gln (Q)   5	  1.6%
Glu (E)  15	  4.9%
Gly (G)  33	 10.7%
His (H)   6	  1.9%
Ile (I)  13	  4.2%
Leu (L)  30	  9.7%
Lys (K)   9	  2.9%
Met (M)   3	  1.0%
Phe (F)   5	  1.6%
Pro (P)  18	  5.8%
Ser (S)  23	  7.4%
Thr (T)  12	  3.9%
Trp (W)   7	  2.3%
Tyr (Y)  15	  4.9%
Val (V)  30	  9.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1490
Hydrogen    H	      2318
Nitrogen    N	       414
Oxygen      O	       447
Sulfur      S	         7

Formula: C1490H2318N414O447S7
Total number of atoms: 4676

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61100
Abs 0.1% (=1 g/l)   1.829, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   1.821, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.77
This classifies the protein as stable.



Aliphatic index: 91.81

Grand average of hydropathicity (GRAVY): -0.131