ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASATFSVAK PSLQGFSEFS GLRNSSALPF AKRSSSDEFV SFVSFQTSAM RSNGGYRKGV 

        70         80         90        100        110        120 
TEAKIKVAIN GFGRIGRNFL RCWHGRKDSP LDVVVINDTG GVKQASHLLK YDSTLGIFDA 

       130        140        150        160        170        180 
DVKPSGDSAL SVDGKIIKIV SDRNPSNLPW GELGIDLVIE GTGVFVDRDG AGKHLQAGAK 

       190        200        210        220        230        240 
KVLITAPGKG DIPTYVVGVN AELYSHEDTI ISNASCTTNC LAPFVKVLDQ KFGIIKGTMT 

       250        260        270        280        290        300 
TTHSYTGDQR LLDASHRDLR RARAAALNIV PTSTGAAKAV ALVLPNLKGK LNGIALRVPT 

       310        320        330        340        350        360 
PNVSVVDLVV QVSKKTFAEE VNAAFRDAAE KELKGILDVC DEPLVSVDFR CSDVSSTIDS 

       370        380        390 
SLTMVMGDDM VKVIAWYDNE WGYSQRVVDL ADIVANNWK 

References and documentation are available.

Number of amino acids: 399

Molecular weight: 42846.83

Theoretical pI: 8.16

Amino acid composition: 
Ala (A)  36	  9.0%
Arg (R)  19	  4.8%
Asn (N)  18	  4.5%
Asp (D)  31	  7.8%
Cys (C)   5	  1.3%
Gln (Q)   8	  2.0%
Glu (E)  13	  3.3%
Gly (G)  33	  8.3%
His (H)   6	  1.5%
Ile (I)  22	  5.5%
Leu (L)  32	  8.0%
Lys (K)  27	  6.8%
Met (M)   6	  1.5%
Phe (F)  16	  4.0%
Pro (P)  14	  3.5%
Ser (S)  37	  9.3%
Thr (T)  22	  5.5%
Trp (W)   5	  1.3%
Tyr (Y)   7	  1.8%
Val (V)  42	 10.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1897
Hydrogen    H	      3038
Nitrogen    N	       526
Oxygen      O	       580
Sulfur      S	        11

Formula: C1897H3038N526O580S11
Total number of atoms: 6052

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38180
Abs 0.1% (=1 g/l)   0.891, assuming all pairs of Cys residues form cystines


Ext. coefficient    37930
Abs 0.1% (=1 g/l)   0.885, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.98
This classifies the protein as stable.



Aliphatic index: 92.33

Grand average of hydropathicity (GRAVY): -0.047