ProtParam
User-provided sequence:
10 20 30 40 50 60
MASDAVKYMP IHGGGTTATT AADIKSFFSA LKPKKTSTFA YAFVITFVSF TLFFAFSPSP
70 80 90 100 110 120
NSSSPWFSNI FTSSSTTTTS DNTSGSQFSS IFSYILPNVT STKPTNRSSD ATDSLSVNAT
130 140 150 160 170 180
SPPLNSNSKN GTLQTPAPET HTPVAKNTTF ESPIVNGTNP DAKNNTSSHP LLSDKSSTTG
190 200 210 220 230 240
SNNQSRTTAD TETVNRNQTT SPAPSKAPVS VDLKTNSSSN SSTASSTPKK QTKTVDLVSS
250 260 270 280 290 300
VKQEIEKWSE SLKNCEFFDG EWIKDDSYPL YKPGSCNLID EQFNCITNGR PDKDFQKLKW
310 320 330 340 350 360
KPKKCSLPRL NGAILLEMLR GRRLVFVGDS LNRNMWESLV CILKGSVKDE TKVYEARGRH
370 380 390 400 410 420
HFRGEAEYSF VFQDYNCTVE FFVSPFLVQE WEIVDKKGTK KETLRLDLVG KSSEQYKGAD
430 440 450 460 470 480
VIVFNTGHWW THEKTSKGED YYQEGSNVYH ELAVLEAFRK ALTTWGRWVE KNVNPAKSLV
490 500 510 520 530 540
FFRGYSASHF SGGQWNSGGA CDSETEPIKN DTYLTPYPSK MKVLEKVLRG MKTPVTYLNI
550 560 570 580 590 600
TRLTDYRKDG HPSVYRKQSL SEKEKKSPLL YQDCSHWCLP GVPDSWNEIL YAELIVKLNQ
LSQTQRKT
References and
documentation are available.
Number of amino acids: 608
Molecular weight: 67925.08
Theoretical pI: 8.94
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 73
Atomic composition:
Carbon C 3019
Hydrogen H 4671
Nitrogen N 813
Oxygen O 943
Sulfur S 15
Formula: C
3019H
4671N
813O
943S
15
Total number of atoms: 9461
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 101800
Abs 0.1% (=1 g/l) 1.499, assuming all pairs of Cys residues form cystines
Ext. coefficient 101300
Abs 0.1% (=1 g/l) 1.491, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.97
This classifies the protein as stable.
Aliphatic index: 62.83
Grand average of hydropathicity (GRAVY): -0.609