ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASEKPQDLM VTCTAPVNIA VIKYWGKRDE ALILPINSSL SVTLHQDQLK TTTTVAISKD 

        70         80         90        100        110        120 
FTEDRIWLNG REEDVGQPRL QACLREIRRL ARKRRSTEDG DTLPLSLSYK VHVASVNNFP 

       130        140        150        160        170        180 
TAAGLASSAA GYACLAYTLA QVYGVEGDLS EVARRGSGSA CRSLYGGFVE WQMGEQADGK 

       190        200        210        220        230        240 
DSIARQIAPE WHWPQLRILI LVVSADKKQT GSTVGMQTSV ETSTLLKFRA ESVVPERMKE 

       250        260        270        280        290        300 
MTRCIQEQDF QGFAQLTMKD SNQFHATCLD TFPPISYLND TSRRIIQLVH RFNTHQGQTK 

       310        320        330        340        350        360 
VAYTFDAGPN AVIFTLEDTV AEFVAAVRHS FPPAANGDKF LKGLQVAPVL LSDELKAALA 

       370        380        390        400 
VEPSPGGVQY IIATQVGPGP QVLDDTHDHL LGQDGLPQRD L 

References and documentation are available.

Number of amino acids: 401

Molecular weight: 44072.02

Theoretical pI: 5.89

Amino acid composition: 
Ala (A)  38	  9.5%
Arg (R)  23	  5.7%
Asn (N)  10	  2.5%
Asp (D)  25	  6.2%
Cys (C)   6	  1.5%
Gln (Q)  26	  6.5%
Glu (E)  21	  5.2%
Gly (G)  27	  6.7%
His (H)   9	  2.2%
Ile (I)  18	  4.5%
Leu (L)  39	  9.7%
Lys (K)  17	  4.2%
Met (M)   7	  1.7%
Phe (F)  14	  3.5%
Pro (P)  20	  5.0%
Ser (S)  27	  6.7%
Thr (T)  29	  7.2%
Trp (W)   5	  1.2%
Tyr (Y)   9	  2.2%
Val (V)  31	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1946
Hydrogen    H	      3090
Nitrogen    N	       546
Oxygen      O	       595
Sulfur      S	        13

Formula: C1946H3090N546O595S13
Total number of atoms: 6190

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41285
Abs 0.1% (=1 g/l)   0.937, assuming all pairs of Cys residues form cystines


Ext. coefficient    40910
Abs 0.1% (=1 g/l)   0.928, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.83
This classifies the protein as unstable.



Aliphatic index: 87.33

Grand average of hydropathicity (GRAVY): -0.227