ProtParam
User-provided sequence:
10 20 30 40 50 60
MASFATKFVI ACFLFFSASA HNVLLPAYGR RCFFEDLSKG DELSISFQFG DRNPQSSSQL
70 80 90 100 110 120
TGDFIIYGPE RHEVLKTVRD TSHGEITLSA PYKGHFQYCF LNENTGIETK DVTFNIHGVV
130 140 150 160 170 180
YVDLDDPNTN TLDSAVRKLS KLTREVKDEQ SYIVIRERTH RNTAESTNDR VKWWSIFQLG
190 200
VVIANSLFQI YYLRRFFEVT SLV
References and
documentation are available.
Number of amino acids: 203
Molecular weight: 23332.33
Theoretical pI: 6.30
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1055
Hydrogen H 1610
Nitrogen N 282
Oxygen O 310
Sulfur S 4
Formula: C
1055H
1610N
282O
310S
4
Total number of atoms: 3261
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23045
Abs 0.1% (=1 g/l) 0.988, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.982, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.32
This classifies the protein as stable.
Aliphatic index: 83.50
Grand average of hydropathicity (GRAVY): -0.220